病毒灭活新鲜血浆在不同时间段质量分析  

Analysis of the quality in virus inactivated fresh plasma during different time period

作　　者：陈素君 谢冬梅 廖丽梅 黄勇华 王艳清 田桂敏 黎文思 邓启健 朱敏捷 

机构地区：[1]清远市中心血站,广东清远511518 [2]清远市清城区源潭医院检验科,广东清远511533

出　　处：《国际检验医学杂志》2016年第4期465-467,共3页International Journal of Laboratory Medicine

基　　金：广东省清远市科技计划课题项目(2013B010)

摘　　要：目的分析亚甲蓝光化学法(MB-P)灭活处理新鲜血浆(简称病毒灭活新鲜血浆)与未经灭活处理的新鲜血浆(简称新鲜血浆)在保存期内不同时间段有效成分的变化,为临床输注提供参考依据。方法取20人份全血(每份400mL),按照标准操作规程制备成新鲜血浆(每份200mL)后,将其一分为二,制备病毒灭活新鲜血浆和新鲜血浆,两种血浆每份无菌分装为5等份,立即放入速冻冰箱速冻,在0(制备后小于72h)、3、6、9、12个月分别检测Ⅴ因子、Ⅷ因子、纤维蛋白原(Fib)、亚甲蓝残留量、总蛋白、pH值、K^+、Na^+、Cl^-水平。结果两种血浆随着保存期的延长,Ⅴ因子、Ⅷ因子、Fib、总蛋白、K^+、Na^+、Cl^-水平呈下降趋势,pH值呈上升趋势,差异有统计学意义(P<0.05)。结论经过病毒灭活过程对血浆Ⅴ因子、Ⅷ因子、Fib、总蛋白、pH值、K^+、Na^+、Cl^-水平均有所影响,但能够达到国家规定的临床用血标准,采用病毒灭活可以在保留血浆基本成分的情况下提高临床用血的安全性。Objective To Analysis of the changes of the effective components of MB-P fresh plasma and fresh plasma in different time in the preservation period,provides the reference for the clinical infusion.Methods Taken 20 doses of whole blood（400mL/copy）,in accordance with standard operating procedures preparation fresh plasma（200mL/copy）;Split them in two parts and divided into virus inactivated plasma and fresh plasma group,and divided this two kinds of plasmar in five equal parts in aseptic packaging each;immediately put them into a refrigerator for quick-frozen.Detected theirⅤ factor,Ⅷ factor,Fib,methylene blue residues,total protein,pH value,the content of K＋,Na＋and Cl-after 0（less than 72 hafter preparation）,3,6,9and 12 months respectively.Results Two kinds of plasma with the extension of the storage period,Ⅴfactor,Ⅷfactor,Fib,total protein,K＋,Na＋,Cl-levels were decreased,pH showed an upward trend,the difference was statistically significant（P0.05）.Conclusion After virus inactivation treatment,the level of plasma constituents have been changed,but to conform to the standard of blood for clinical use.Therefore,the virus inactivation treatment could be used to improve the safety of the effective components of plasma.

关 键 词：新鲜血浆 病毒灭活 质量 时间段 

分 类 号：R457.14[医药卫生—治疗学]