醒鼻凝胶剂对变应性鼻炎豚鼠核转录因子-κB信号通路的影响  

Effects of Xingbi Gels on NF-κB signal pathway in allergic rhinitis guinea pigs

作　　者：南丽红[1] 向青[1] 徐伟[1] 李煌[1] 褚克丹[1] 郑健[1] 黄枚[1] 

机构地区：[1]福建中医药大学药学院,福州350122

出　　处：《中华中医药杂志》2016年第2期441-444,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81073117);福建省科技厅指导性自筹项目(No.2012D010)~~

摘　　要：目的:观察醒鼻凝胶剂对变应性鼻炎(AR)豚鼠鼻黏膜组织中核转录因子-κB(NF-κB)和血清中白介素-5(IL-5)、粒细胞-巨噬细胞集落刺激因子(GM-CSF)、趋化因子CCL-1表达的影响,从而探讨醒鼻凝胶剂治疗AR的可能机制。方法:健康豚鼠60只随机分为6组:正常对照组,模型组,布地奈德组,醒鼻凝胶剂高、中、低剂量组。除正常对照组外,其他各组均以卵清蛋白加氢氧化铝腹腔注射制备AR豚鼠模型,于造模第16天开始给药,2次/d,连续给药11d后,ELISA法测定各组豚鼠血清中IL-5、GM-CSF、CCL-1的含量,RT-PCR、免疫组化法分别检测各组豚鼠鼻黏膜组织中NF-κB m RNA和NF-κB p65蛋白的表达。结果:模型组鼻黏膜组织中NF-κB m RNA和NF-κB p65蛋白的表达及血清中IL-5、GM-CSF、CCL-1的含量均明显高于正常对照组(P<0.01),且NF-κB p65的激活与IL-5、GM-CSF、CCL-1的表达呈显著正相关。AR模型豚鼠滴鼻给予高剂量醒鼻凝胶剂后鼻黏膜组织中NF-κB m RNA和NF-κB p65蛋白的表达明显减少(P<0.01),血清中IL-5、GM-CSF、CCL-1的含量均明显降低(P<0.01)。结论:醒鼻凝胶剂可能通过抑制NF-κB的活化与核移位,减弱了IL-5、GM-CSF、CCL-1的表达,从而减轻鼻道的炎性反应达到治疗作用。Objective：To observe the influence of Xingbi Gels on expression of NF-κB in nasal mucosa and expression of interleukin-5（IL-5）,granulocyte macrophage colony stimulating factor（GM-CSF） and chemokine CCL-1 in serum of allergic rhinitis（AR） guinea pigs,in order to explore the therapeutic mechanism of Xingbi Gels on AR.Methods：Sixty healthy guinea pigs were randomly divided into 6 groups：control group,model group,budesonide group,low,middle,and high dosage of Xingbi Gels groups.All the guinea pigs except control group received intraperitoneal injecting with ovalbumin and aluminium hydroxide in order to establish the guinea pigs model of AR.The treatments were started on the 16 th day after model establishing and lasted for 11 days.The contents of IL-5,GM-CSF and CCL-1 in serum were examined by using ELISA.The expression of NF-κB p65 protein and NF-κB mRNA in the nasal mucosa was detected by using immunohistochemical technique and RT-PCR respectively.Results：The expression of NF-κB mRNA and NF-κB p65 protein in nasal mucosa and contents of IL-5,GM-CSF and CCL-1 in serum of the guinea pigs in model group were significantly higher than those of control group（P〈0.01）,and the activation of NF-κB p65 was positively correlated with the expression of IL-5,GM-CSF and CCL-1 in serum.After treating with high dosage of Xingbi Gels,the expression of NF-κB mRNA and NF-κB p65 protein in the nasal mucosa and the contents of IL-5,GM-CSF and CCL-1in serum of AR guinea pigs were decreased remarkably（P〈0.01）.Conclusion：The Xingbi Gels may decrease the expression of IL-5,GM-CSF and CCL-1 by inhibiting the activation and unclear translocation of NF-κB,so as to reduce the inflammation of nasal passage and play the role in treating AR.

关 键 词：醒鼻凝胶剂 变应性鼻炎 豚鼠 炎性因子 核转录因子-ΚB 

分 类 号：R285.5[医药卫生—中药学]