基于HPLC的不同肾茶种质质量评价  

Quality evaluation of Clerodendranthus spicatus of different germplasms based on HPLC method

作　　者：李戈[1] 王艳芳[1] 赵俊凌[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所云南分所,云南景洪666100

出　　处：《中华中医药杂志》2016年第2期630-633,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：中国医学科学院药用植物研究所创新团队研究计划(No.121306)~~

摘　　要：目的:研究不同来源肾茶种质中化学成分的差异。方法:采用HPLC法测定肾茶中主要有效成分迷迭香酸与熊果酸的含量,并运用《中药色谱指纹图谱相似度评价系统(2004A)版》软件分析不同产地来源肾茶的HPLC指纹图谱。结果:15份不同种质来源肾茶中迷迭香酸含量为0.03%-0.69%,熊果酸含量为0.22%-0.41%;15份肾茶样品中有20个共有指纹峰,它们指纹图谱与对照指纹图谱的整体相似度在0.874-0.998。结论:肾茶种质的质量评价能够为其优良品种选育提供理论依据。Objective：To study the difference in chemical composition among Clerodendrou spicatus with different germplasms.Methods：The content of rosmarinic acid and ursolic acid in Clerodendrou spicatus were determined by using HPLC,as well as the fingerprint of Clerodendrou spicatus with different districts were analyzed by using Similarity Evaluation System for Chromatographic Fingerprint of CMM（2004A）.Results：The contents of rosmarinic acid and ursolic acid in 15 samples with different germplasms were 0.03%-0.69%and 0.22%-0.41%,respectively.There were twenty common fingerprint peaks among the 15 samples,and the overall similarity between finger-print and control finger-print was within the range of 0.874-0.998.Conclusion：The quality evaluation of Clerodendranthus spicatus of different germplasms could provide the theoretical basis for breeding of new variety.

关 键 词：肾茶 迷迭香酸 熊果酸 指纹图谱 

分 类 号：R284.1[医药卫生—中药学] O657.72[医药卫生—中医学]