乌腺金丝桃总黄酮对大鼠心肌细胞心律失常模型内向整流钾电流的影响  

Influence of total flavonoids from Hypericum attenuatum Choisy on inward rectifier potassium current of rat cardiac muscle cell arrhythmia model

作　　者：王艳丽[1,2] 马育轩[2] 高彦宇[2] 李冀[2] 

机构地区：[1]黑龙江护理高等专科学校,哈尔滨150086 [2]黑龙江中医药大学,哈尔滨150040

出　　处：《中华中医药杂志》2016年第2期656-658,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81173185);黑龙江省自然科学基金重点项目(No.ZD201111);黑龙江中医药大学优秀领军人才支持计划(No.2012)~~

摘　　要：目的:探讨乌腺金丝桃总黄酮对大鼠心肌细胞心律失常模型内向整流钾电流(IK1)的影响。方法:应用全细胞膜片钳技术观察乌腺金丝桃总黄酮对大鼠心肌细胞心律失常模型IK1电流密度峰值、Ⅰ-Ⅴ曲线的影响。结果:乌头碱使正常心肌细胞IK1内向电流密度峰值降低,Ⅰ-Ⅴ曲线内向部分明显上移;乌腺金丝桃总黄酮使模型细胞IK1内向电流密度峰值升高,Ⅰ-Ⅴ曲线内向部分明显下移。结论:乌腺金丝桃总黄酮对乌头碱致不同去极化水平的模型细胞IK1内向电流减弱有非常明显的恢复作用,其抗心律失常的机制可能与增强IK1有关。Objective：To investigate the influence of total flavonoids from Hypericum attenuatum Choisy on inward rectifier potassium current（IK1） of rat cardiac muscle cell models arrhythmia.Methods：The effects of total flavonoids from Hypericum attenuatum Choisy on the peak current density and Ⅰ-Ⅴ curve of IK1 in the model cells were observed by using whole-cell patch clamp technique.Results：Total flavonoids from Hypericum attenuatum Choisy could increase the IK1 peak current density and recover the IK1 Ⅰ-Ⅴ curve of the model cells which induced by aconitine.Conclusion：The total flavonoids from Hypericum attenuatum Choisy could recover the weakening inward current of IK1 in model cells caused by aconitine,and its antiarrhythmic mechanism might be related to the enhancement of IK1.

关 键 词：心肌细胞 心律失常 乌腺金丝桃总黄酮 内向整流钾电流 

分 类 号：R285.5[医药卫生—中药学]