蛋鸽雌激素受体基因多态性与产蛋量关联分析  

Association Analysis Between the Single Nucleotide Polymorphism of ESR Genes and Egg Production in Laying Pigeon(Columba livia)

作　　者：陶争荣[1] 徐小钦[1,2] 董丽艳[3] 钟声亮 卢立志[1] 

机构地区：[1]浙江省农业科学院畜牧兽医研究所,杭州310021 [2]南京农业大学,南京210095 [3]温州科技职业学院,温州325006 [4]星亮鸽业有限公司,平阳325400

出　　处：《农业生物技术学报》2016年第3期373-379,共7页Journal of Agricultural Biotechnology

基　　金：浙江省"十二五"育种专项(No.2012C12906-18);国家科技部富民强县专项子课题(No.GKZ201406);国家科技部富民强县专项子课题(No.GKZ201402);湖州市攻关计划农业项目(No.2014GN02)

摘　　要：雌激素受体(estrogen receptor,ESR)是核受体超家族中的一种配体依赖的转录因子,大量研究显示,ESR基因与动物的繁殖活动密切相关。为了分析ESR基因单核苷酸多态性(single nucleotide polymorphism,SNPs)及其与蛋鸽(Columba livia)产蛋量的关系,本研究以泰平王鸽(C.livia)215个样本为研究对象,采用第一代测序法对ESR基因部分片段进行SNPs筛选。在所测的3个片段中共发现了10个突变位点,分别为ESRα基因的T129416C,ESRβ基因的T365C、A388G、C389A、A429G、G451A、G577A、T672C、A923C和T17167C,其中有3个突变位于外显子上,但只有位点G577A导致了氨基酸的改变,由氨基酸异亮氨酸(isoleucine,Ile)转变为缬氨酸(valine,Val)。对10个位点进行了Hardy-Weinberg平衡状态检验,结果显示,所有位点均处于平衡状态。10个位点的连锁不平衡分析显示,位点S2与位点S4、S9为强连锁不平衡(D′≈1,r2>0.8),位点S4、S9可以完全代表S2。多态位点与产蛋量关联分析显示,位点S8与产蛋量呈极显著相关,其中杂合子AB型的平均产蛋量极显著的高于纯合子AA型(P<0.01);而纯合子BB型的平均产蛋量低于AB型,高于AA型,但差异不显著(P>0.05)。本研究表明,ESRβ基因中位点S8可作为蛋鸽产蛋量性状选育的候选分子标记位点,为蛋鸽的分子标记辅助育种提供资料。Estrogen receptor（ESR） is a ligand-dependent transcription factor of nuclear receptor superfamily.Numerous studies showed that ESR genes areclosely related to animal reproduction activities. In order to analyze the correlation between the single nucleotide polymorphisms（SNPs） of ESR genes and eggproduction trait of laying pigeon（Columba livia）, the SNPs of partial ESR gene fragments were screened by the first- generation sequencing in 215 Taiping King pigeons（C. livia）. Ten SNPs were detected in the threefragments, T129416 C was located in intron 6 of ESRα gene, the other nine SNPs were detected in ESRβ.They were T365 C, A388 G, C389 A and A429 G in intron 1, G451 A, G577 Ain exon 2, T672 C and A923 C inintron 3, and T17167 C in exon 5 of ESRβ gene. Among these ten sites, three of the mutations were located in exon, but only G577 A made amino acid change from isoleucine（Ile） to valine（Val）. These two amino acids are non-polar and hydrophobic, it is a common missense mutation that has little effect on protein polarity in vivo. All the ten sites were in balance based on the Hardy-Weinberg equilibrium test. The results of Linkage disequilibrium analysis among the ten sites showed that the sites S1 and S10 were not meaningful linkage disequilibrium relative to others, because there were greater physical distances between the two sits and the others. The sites between S2 and S4, S9 were strong linkage disequilibrium（D′ ≈1 and r^2〉0.8） and site S2 could be completely represented by S4 or S9. The correlation analysis between SNPs of ESR genes and egg production showed that only site S8 was significantly correlated to egg production, genotype AB of this site was significantly higher than AA in egg production（P〈0.01）, while BB genotype was higher than AA genotype and lower than AB genotype, but the differences were not significant（P〉0.05）. The results indicate that the S8 site of ESRβ gene can be used as a molecular marker for egg-production traits in MAS breeding of layin

关 键 词：雌激素受体(ESR) 蛋鸽 单核苷酸多态性(SNPs) 产蛋量 

分 类 号：S836[农业科学—畜牧学]