基于线粒体COI和Cytb基因的中国灰蝶三亚科主要类群间的系统发育关系分析  

Phylogenetic relationship within and between three major subfamilies of the Chinese lycaenids(Lepidoptera: Lycaenidae) based on mitochondrial COI and Cytb gene sequences

作　　者：夏雪琴[1] 陈晓[1] 夏琛琛 石庆会[1] 郝家胜[1] 

机构地区：[1]安徽师范大学生命科学学院分子进化与生物多样性研究室,安徽芜湖241000

出　　处：《昆虫学报》2016年第1期77-84,共8页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(41172004)

摘　　要：【目的】针对中国灰蝶科中亲缘关系较近的3个主要亚科[灰蝶亚科(Lycaeninae)、线灰蝶亚科(Theclinae)以及眼灰蝶亚科(Polyommatina)],基于线粒体基因序列数据研究它们主要类群间的系统发育关系。【方法】对3亚科共53种灰蝶的线粒体COI和Cytb基因进行序列测定和序列变异分析,同时,基于最大似然法(maximum likelihood,ML)和贝叶斯法(bayesian inference,BI)等建树方法重建53种灰蝶的系统发育树。【结果】串联的2个基因共1 431 bp,其中保守位点855个,可变位点576个,简约信息位点488个;A+T的平均含量为74.5%,明显高于G+C的平均含量(25.5%)。系统树显示,灰蝶亚科以及眼灰蝶亚科均是单系发生,线灰蝶亚科则为并系群。全部灰蝶物种共分为三大支系:灰蝶亚科为第1支系;眼灰蝶亚科与线灰蝶亚科中的旖灰蝶族(Hypolycaenini)、富丽灰蝶族(Aphnaeini)分别构成单系群并互为姊妹群,它们共同构成第2支系;线灰蝶亚科中的美灰蝶族(Eumaeini)、玳灰蝶族(Deudorigini)、娆灰蝶族(Arhopalini)和线灰蝶族(Theclini)构成第3支系,其亲缘关系为:(((线灰蝶族+娆灰蝶族)+玳灰蝶族)+美灰蝶族)。【结论】本研究涉及的3个灰蝶亚科中,灰蝶亚科是一个独立的支系,眼灰蝶亚科与线灰蝶亚科之间有较近的亲缘关系,但它们内部主要类群间的系统发育关系还需要进一步的研究。[ Aim] To explore the phylogenetic relationships within and between three major subfamilies （Polyommatinae, Theclinae and Lycaeninae） of the Chinese lycaenids based on the mitochondrial gene sequence data. [ Methods ] The partial mitochondrial CO1 and Cytb genes of 53 species were amplified and determined with PCR method, the sequence variation of these species were also analyzed, and the phylogenetic trees of these lycaenid species were reconstructed with maximum-likelihood （ML） and Bayesian-inference （BI） methods. [ Results ] The results of the sequence analysis showed that the two combined genes are 1 431 bp in length, including 855 conserved sites, 576 variable sites and 488 parsimonious-informative sites, and the average percentage of A ＋ T is 74.5%, which is much higher than that of G ＋ C （25.5%）. The results of phylogenetic analyses indicated that the Polyommatinae and Lycaeninae were monophyletic groups, whereas the Theclinae was a paraphyletic group. All the taxa in this study contained three main clades： the first was the subfamily Lycaeninae, the second was the subfamily Polyommatinae plus the tribes Hypolycaenini and Aphnaeini of the subfamily Theclinae, and the third was the other four tribes of the subfamily Theclinae, with their relationship as （ （ （ Theclini ＋ Arhopalini） ＋ Deudorigini） ＋ Eumaeini）. [ Conclusion ] The subfamily Lycaeninae is a monophyletic group, while the Polyommatinae and Theclinae are more closely to each other, with the relationship of their main groups awaiting further investigation.

关 键 词：鳞翅目 灰蝶科 COI基因 CYTB基因 系统发育 中国 

分 类 号：Q969[生物学—昆虫学]