加味六安煎对咳嗽变异性哮喘大鼠肿瘤坏死因子-α和白细胞介素-5的影响  

The effect of Jiawei Liuan decoction on TNF-α and IL-5 in cough variant asthma rats

作　　者：霍婧伟[1] 魏丽娜[2] 苟旭蕾 张媛[2] 王东升[2] 吴力群[1] 

机构地区：[1]北京中医药大学东方医院儿科,100078 [2]北京中医药大学第二临床医学院

出　　处：《环球中医药》2016年第1期6-9,共4页Global Traditional Chinese Medicine

基　　金：北京中医药大学自主选题(2013-QNJSZX018)

摘　　要：目的探讨加味六安煎治疗咳嗽变异性哮喘(cough variant asthma,CVA)的作用机制。方法将60只Wistar大鼠随机分为正常对照组、模型组、孟鲁司特钠组、加味六安煎低、中、高剂量组。以卵蛋白、氢氧化铝致敏并吸入卵蛋白激发法复制咳嗽变异性哮喘大鼠模型,观察各组大鼠咳嗽次数、血清肿瘤坏死因子-α(tumor necrosis factor alpha,TNF-α)、白细胞介素-5(interleukin-5,IL-5)的含量及肺泡灌洗液中嗜酸性粒细胞(eosinophile,EOS)计数。结果 (1)咳嗽次数:CVA模型组较其余各组咳嗽次数明显增加(P<0.001);与CVA模型组相比,各治疗组咳嗽次数明显减少(P<0.05);(2)EOS计数:与正常组比较,其余各组支气管肺泡灌洗液EOS计数明显升高(P<0.05);(3)TNF-α值:CVA模型组与正常组比较,血清TNF-α值明显增高(P<0.05),高剂量组与孟鲁司特钠组比较,无显著性差异(P>0.05);(4)IL-5值:CVA模型组血清IL-5值较其余各组明显升高(P<0.05);其中高剂量组血清IL-5值较其余各治疗组比较降低最明显(P<0.05)。结论加味六安煎对咳嗽变异性哮喘大鼠有较好的治疗作用,其机制可能是通过降低CVA大鼠血清TNF-α、IL-5水平,改善CVA大鼠咳嗽症状、降低EOS数量,从而达到治疗CVA的目的。Objectives To obserle the effect of Jiawei Liuan decoction on blood serum lelel of TNF-α and IL-5, and eosinophil （ EOS） lelel in bronchoalleolar lalage fluid （ BALF） in cough lariant asthma （CVA） rats, thereby determining the mechanism of Jiawei Liuan decoction in CVA treatment. Methods 60 Wistar rats are randomly allocated into normal control group （ NCG） , CVA group （ CVAG） , montelukast sodium group （ MSG ） , Jiawei Liuan low dose group （ LALG ） , Jiawei Liuan medium dose group （ LAMG ） and Jiawei Liuan high dose group （ LAHG ） . CVA rat models are hypersensitized by albumin and aluminium hydroxide. The rats are made to inhale albumin to actilate the replication of CVA rat models. Cough frequencies, blood serium TNF-α and IL-5 lelels, as well as BALF EOS lelels are obserled. Results Cough frequency：CVAG experienced increased cough frequency as compared to other groups （P〈0. 001）, cough frequencies of all groups are significantly lower as compared to CVAG（P〈0. 05）. Eosinophil count：BALF EOS of all groups are significantly increased as compared to NCG （P〈0. 05）. TNF-α lelels： CVAG blood serium TNF-α is significantly increased （P〈0. 05）, there is no significant difference between LAHG and MSG （P〉0. 05）. IL-5 lelels： CVAG blood serum IL-5 are sig-nificantly increased as compared to other groups （P〈0. 05）, IL-5 blood serum was significantly decreased in LAHG as compared to other trearment groups （P〈0. 05）. Conclusions Jiawei Liuan decoction has prominent therapeutic effect for CVA rats. The mechanism inlolled could be that it is able to decrease the blood serum TNF-α, IL-5 lelels, decrease cough symptoms and decrease BALF EOS lelel in CVA rats in order to achiele therapeutic effect.

关 键 词：加味六安煎 咳嗽变异性哮喘 肿瘤坏死因子-Α 白细胞介素-5 

分 类 号：R285.5[医药卫生—中药学]