补虚化瘀法对豚鼠形觉剥夺性近视模型多焦视网膜电图的影响  

Influences of traditional Chinese medicine of Buxu Huayu on the multifocal electroretinogram of a model of form deprivation myopia in guineas

作　　者：杨婷[1] 莫亚[2] 陈莉苹[3] 黄秀蓉[2] 曹斌[1] 

机构地区：[1]成都中医药大学研究生院,610072 [2]成都中医药大学附属医院眼科 [3]天津医科大学总医院滨海医院眼科

出　　处：《环球中医药》2016年第1期15-20,共6页Global Traditional Chinese Medicine

基　　金：四川省教育厅课题(11ZA065);四川省卫生厅课题(110527);成都中医药大学附属医院科技发展基金(2012-D-YY-12)

摘　　要：目的通过观察补虚化瘀法对豚鼠形觉剥夺性近视(form deprivation myopia,FDM)多焦视网膜电图(multifocal electroretinogram,mf ERG)的影响,探讨具有补虚化瘀功效的中药制剂八珍颗粒是否对形觉剥夺性近视豚鼠视网膜功能有影响。方法将16只3周龄豚鼠随机分为两组:模型组、补虚化瘀组,每组各8只,右眼为剥夺眼,采用半透明眼罩遮盖诱导形觉剥夺性近视,左眼不予处理,为对照眼。补虚化瘀组予八珍颗粒混悬液1.12 g/(kg·d),每天同一时间灌胃1次,模型组予蒸馏水1 m L灌胃1次。所有动物在造模前与造模4周后进行带状检影,造模后进行多焦视网膜电图检查。结果 (1)经过4周形觉剥夺,豚鼠右眼向近视转变,与造模前比较,豚鼠右眼近视屈光度有显著改变(P<0.05),而模型组右眼屈光度与补虚化瘀组右眼屈光度比较无明显差异(P>0.05)。(2)形觉剥夺4周后,模型组右眼1、2环N1波峰时,1、2环P1峰时较模型组左眼延迟,差异有统计学意义(P<0.05);模型组右眼总波、1、2、3环N1波反应密度,总波、1、2、3、4环P1波反应密度较模型组左眼显著降低,差异有统计学意义(P<0.05);补虚化瘀组右眼1环N1波峰时,总波、2环P1波峰时较模型组右眼延迟,差异有统计学意义(P<0.05)。中药制剂八珍汤颗粒组右眼总波、2、3环N1波反应密度,总波、1、2、3、4环P1波反应密度均较模型组右眼有所增加,差异有统计学意义(P<0.05)。结论补虚化瘀法有助于形觉剥夺性近视豚鼠视网膜mf ERG反应密度、峰时及其功能的恢复。Objective To obserle the effect of traditional Chinese medicine （ TCM ） of Buxu Huayu on the guinea of form deprilation myopia （ FDM ） , and obserle the effect of traditional Chinese medicine （ TCM） of Bazhen granules on the retinal function of guinea of FDM. Methods 16 guineas were randomly dilided into 2 groups： model control group （ n=8 ） and Bazhen granules group （ n=8 ） , eight guineas each group. The right eyes of all guineas were form-depriled with semi-transparent membraes for 4weeks, and the left eyes were used as normal control without any treatment. Bazhen granules suspension1. 12 g/（ kg·d） was gilen for consecutile 4 weeks in the guineas of Buxu Huayu group,and distilled water was gilen for consecutile 4 weeks in the guineas of model control group. Before and 4 weeks after form dep-rilation, refraction was measured by retinoscopy after cycloplegia. after gilen drugs or distilled water for 4 weeks, to obserle the effection by multifocal electroretinogram （ mf ERG） . Results After 4 weeks of form deprilation,the guinea pigs＇ s right eyes had myopic shift, and there was an approximately increase in FDM myopic refraction comparing to pre-models building;But the refraction was no oblious change between model control group and Bazhen decoction group after models builded in 4 weeks （P〉0. 05）. The peak time of the N1 in the ring1-2 and the P1 in the total wale and ring1-2 in the model group＆#39; s right eyes was statistically increased compared with that of the left eyes of the model group （ P〈0. 05 ） . The amplitude densities of the N1 in the total wale ,ring1-3 and the P1 in the total wale, ring1-4 in right eyes of model group was statistically decreased compared with that of left eye in the model group（P〈0. 05）. The peak time of the N1 in the ring1 and the P1 in the total wale and ring 2 in the right eyes of Buxu Huayu group was significantly decreased compared with that of right eye in the model group（P〈0. 05）. The amplitude densities of the N1 in

关 键 词：补虚化瘀 豚鼠形觉剥夺近视模型 多焦视网膜电图 

分 类 号：R285.5[医药卫生—中药学]