不同OTUs划分阈值对湖泊浮游细菌α多样性的影响  

Effects of Different OTUs Cutoff Values onα-Diversity of Bacterioplankton in Lake

作　　者：沈烽[1,2] 黄睿[1,2] 王司辰 曹新益 赵大勇[1,2] 

机构地区：[1]河海大学水文水资源与水利工程科学国家重点实验室,江苏南京210098 [2]河海大学水文水资源学院,江苏南京210098

出　　处：《化学与生物工程》2016年第2期25-30,共6页Chemistry & Bioengineering

基　　金：国家自然科学基金资助项目(41371098;41571108);江苏省自然科学基金资助项目(BK20151614);中央高校基本科研业务费专项资金资助项目(2015B14214;2015B31714)

摘　　要：微生物研究中,湖泊浮游细菌微生物群落的α多样性通过OTUs（operation taxonomy units）来分析评价。考察了现有手段下不同OTUs划分阈值或分类单元＂属＂水平对α多样性大小和趋势的影响,并推断了分类单元＂属＂所对应的OTUs划分阈值。通过454高通量测序技术研究了南京莫愁湖、前湖、玄武湖、紫霞湖4个湖泊的浮游细菌微生物群落,探讨了不同OTUs划分阈值对α多样性指数OTUs、Chao1、Invsimpson、Shannon的影响。结果发现,OTUs划分阈值不同,OTUs和Chao1指数变化一致,Invsimpson和Shannon指数变化一致;在不同的OTUs划分阈值下,4个湖泊的OTUs和Chao1指数在不同季节的排序基本保持不变,Invsimpson和Shannon指数在不同季节的排序发生改变。表明,分类单元＂属＂所对应的OTUs划分阈值在0.06~0.08范围内;原有的以0.03或0.1阈值划分OTUs来分析α多样性是合理的;所用的分析微生物群落α多样性方法是全面的、可靠的。Theα-diversity of bacterioplankton community in lake is generally evaluated based on OTUs（operation taxonomy units）.This study investigated the effects of different OTUs cutoff values or genus level onα-diversity and detected the approximation of OTUs cutoff values which was corresponding to taxonomic level of genera for bacteria.By 454high-throughput sequencing technology,the bacterioplankton communities of Mochou Lake,Qian Lake,Xuanwu Lake,and Zixia Lake in Nanjing city were studied.Different OTUs cutoff values were assumed to explore the variation of OTUs,Chao1,Invsimpson,Shannon indexes.Results showed that,OTUs and Chao1 indexes showed a consistent change with the variation of OTUs cutoff values,while Invsimpson and Shannon indexes showed a consistent change.Along with the distribution of OTUs cutoff values,OTUs and Chao1 indexes of four lakes remained monotonous compared to each other in different seasons,whereas the results of Invsimpson and Shannon indexes differed with fluctuated values.The taxonomic level of genera was roughly corresponding to differentiation threshold of 0.06~0.08.The well-known thresholds of 0.03 or 0.1as cutoffs to analyze microbial sequenceα-diversity was reasonable.Method for analyzingα-diversity of the microbial community was comprehensive and reliable.

关 键 词：Α多样性 高通量序列 OTUs 浮游细菌 16SrRNA 

分 类 号：Q93[生物学—微生物学] R318[医药卫生—生物医学工程]