冠心病“毒证”与传统外科“毒证”蛋白质组学差异研究  

Study on Specific Proteins Between Toxin Syndrome in Coronary Heart Disease and Traditional Toxin Syndrome in Surgical Diseases

作　　者：许京锋[1] 尚青华[1] 王欣[1] 徐浩[1] 

机构地区：[1]中国中医科学院西苑医院,北京100091

出　　处：《中西医结合心脑血管病杂志》2016年第1期4-7,共4页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：973计划专项课题:心血管血栓性疾病"瘀毒"病因学说的系统研究(No.2006CB504803);国家自然基金课题:基于蛋白质组学冠心病血瘀证瘀毒病机转变的生物学基础研究(No.30973702)

摘　　要：目的通过对中医传统"毒证"和冠心病(CHD)"毒证"的组间比较,探讨冠心病"毒证"的特异蛋白表达,为冠心病"毒证"理论的生物学基础提供依据。方法选择急性心肌梗死(AMI)、传统外科"毒证"病人各15例,采用Matrix Assisted Laser Desorption Ionization/Time-of-Flight Mass Spectroscopy(Maldi-Tof-MS)方法对两组人群血清蛋白进行比较分析和鉴定,最终确定CHD"毒证"和传统毒证的差异蛋白。结果共找到4个有明显统计学意义的蛋白多肽链,其中分子量为2 022.61Da的多肽链LOC653879(补体3类似物,Similar to Complement C3)组间差异最大,且能很好地将这两组区分;用QC方法建立的模型示该蛋白多肽链能够很好地与其他蛋白相结合,得到的识别率为93.33%。结论补体C3类似物LOC653879是区别冠心病"毒证"与传统外科"毒证"的关键蛋白,值得进一步研究。Objective To find the specific proteins between toxin syndrome in coronary heart disease（CHD）and traditional toxin syndrome in surgical diseases. Methods We included 30 patients in 2 different groups including acute myocardial infarction（AMD and traditional toxic syndrome（15 patients in each group）.Matrix assisted laser desorption ionization/time- of- flight mass spectroscopy （Maldi - Tof - MS）technology was used to analyze and identify the differential proteins. Results Four peptides were found for distinguishing toxin syndrome in CHD and traditional toxin syndrome, peptides significantly higher expression in CHD toxin were 2 022. 61Da（LOC653879, similar to complement C3）was identified, and it can distinguish the two group very well. Conclusion Similar to Complement C3 LOC653879 were identified as key protein distinguishing CHD blood - stasis toxin syndrome and traditional toxin syndrome, and it should be studied further.

关 键 词：冠心病 毒证 蛋白组学 

分 类 号：R259[医药卫生—中西医结合]