48株大肠杆菌超广谱β-内酰胺酶基因型检测  

Detection of extended-spectrum β-lactamases genotype among 48 Escherichia coli

作　　者：林雅[1] 简燕娟 方志超[1] 范才良 程方俊[1] 

机构地区：[1]西南大学荣昌校区动物医学系,重庆荣昌402460 [2]重庆市大足区宝兴镇人民政府,重庆大足402373 [3]重庆市荣昌县畜牧兽医局,重庆荣昌402460

出　　处：《中国兽医杂志》2016年第1期100-102,共3页Chinese Journal of Veterinary Medicine

基　　金：西南大学博士启动基金2013BSr03

摘　　要：为了解重庆地区部分鸭场大肠杆菌中超广谱β-内酰胺酶(ESBLs)基因分布情况及抗生素的应用对鸭场环境菌的影响。本试验采用PCR技术,对48株大肠杆菌(包括20株病料分离株和28株鸭场土壤分离株)的ESBLs基因TEM、SHV、CTX-M进行检测。结果表明,TEM、SHV、CTX-M型基因在病料分离株中的检出率分别为100%、30%、25%,鸭场土壤中的检出率分别为57.1%、21.4%、28.6%。不同来源的菌株均能检测出耐药基因,且部分细菌携带两种以上耐药基因;病料分离株和鸭场土壤分离株中携带的ESBLs基因均以TEM型为主。The purpose of this study was to detect genotype of ESBLs in E.coli isolated from duck and farm in Chongqing, and the influence of antibiotics on the environmental E.coli in farm. PCR amplifications was applied to detect the β-1actam resistant genotypes TEM, SHV, and CTX-M of extended-spectrum β-lactamases （ESBIs） from 48 isolates, 20 isolated from duck and 28 isolated from farm.The results showed that the detection rates of drug resistant genotypes of TEM, SHV, and CTX-M from epidem- ic materials were 100%, 30%, and 25% respectively, and those from soil were 57.1%, 21.4%, and 28.6%, respectively. In conclu- sion, drug resistant genotypes or composite genotypes can be detected from strains of different sources, and the major ESBLs geno- type was TEM.

关 键 词：鸭源大肠杆菌 超广谱Β-内酰胺酶 耐药基因 

分 类 号：S852.61[农业科学—基础兽医学]