海滨斯氏线虫生物学特性研究  

Study on the Biological Characteristics of Steinernema litorale

作　　者：刘瑞[1] 刘奇志[1] 

机构地区：[1]中国农业大学植物保护学院,北京100193

出　　处：《中国生物防治学报》2016年第1期101-105,共5页Chinese Journal of Biological Control

基　　金：国家公益性行业(林业)科研专项(201004037);国家科技支撑计划(2014BAD20B01)

摘　　要：研究旨在明确采自北京延庆县杏树根际土壤中海滨斯氏线虫Steinernema litorale的生物学特性,进而明确开发应用的潜能。结果表明:在侵染24 h、剂量640 IJs/幼虫下,大蜡螟校正死亡率达76.7%;48 h时,除10和20 IJs/幼虫外,40、80、160、320和640 IJs/幼虫剂量下大蜡螟的校正死亡率均为100.0%。在侵染36 h,剂量160、320和640 IJs/幼虫下,黄粉虫校正死亡率均达50.0%以上;48 h时,剂量640 IJs/幼虫下,黄粉虫校正死亡率为100.0%。经过50.5%RH下干燥9 h后,海滨斯氏线虫存活率仍达94.0%。线虫在30℃条件下的水平运动能力强于18和25℃下,24 h后有62.7%爬行3.6 cm寻找到寄主体液。水浴6 h时,30、32、34、35℃下线虫的死亡率均在50.0%以上;38℃水浴2 h后,线虫的死亡率为100.0%。研究结果表明,海滨斯氏线虫具有很高的侵染力和长时间的耐干燥力,显示了其防治害虫的开发利用潜能。The biology analysis on Steinernema litorale, isolated from the apricot tree soil in Yanqing district of Beijing, was studied to understand the potential of being bio-control agent. The results showed that when larvae were exposed to 640 infective juveniles（IJs）/larva for 24 h, 76.7% mortality of Galleria mellonella were recorded. The corrected mortality of G. mellonella was 100.0% at dosages of 40, 80, 160, 320, 640 IJs/larva, except of the dosages of 10 and 20 IJs/larva after 48 h. The corrected mortality of Tenebrio molitor was more than 50% at the dosages of 160, 320 and 640 IJs/larva after 36 h, and 100.0% at the dosages of 640 IJs/larva after 48 h. The survival rate of S. litorale was still up to 94.0% at 50.5% RH desiccated for 9 h. S. litorale ability of transverse diffusion at 30 ℃ was better than that at 18 and 25 ℃. There was 62.7% individuals could move 3.6 cm in 24 h to reach the host at 30℃. More than 50.0% mortality rate of S. litorale was obtained in water bath at 30, 32, 34 and 35 ℃ for 6 h, 100.0% at 38 ℃for 2 h. According to the results, S. litorale has high infectivity and long resistance desiccation capacity, which shown its potential of development and application to control pest.

关 键 词：海滨斯氏线虫 侵染力 耐干燥 耐热性 水平运动能力 

分 类 号：S476[农业科学—农业昆虫与害虫防治]