家兔血浆中地克珠利提取方法的比较研究  

Comparative Research of Extraction Methods for Diclazuril in Rabbit Plasma

作　　者：杨丽萍[1] 姜文学[1] 高淑霞[1] 张秀玲[1] 孙海涛[1] 王文志[1] 白莉雅[1] 

机构地区：[1]山东省农业科学院畜牧兽医研究所/山东省畜禽疫病防治与繁育重点实验室,山东济南250100

出　　处：《山东农业科学》2016年第2期125-128,共4页Shandong Agricultural Sciences

基　　金：国家兔产业技术体系项目(编号:CARS-44-B-5)

摘　　要：为确定适合的家兔血浆中地克珠利提取方法,比较了甲醇或乙腈沉淀法、乙酸乙酯萃取法、DMF及乙腈联合沉淀法提取家兔血浆中地克珠利的效果。结果表明:采用DMF及乙腈联合沉淀法时,血浆中地克珠利的浓度与响应值线性关系好,且提取回收率>85%,该方法适于提取家兔血浆中的地克珠利。To establish the extraction method of diclazuril in rabbit plasma,the effects were compared among the methods of methanol / acetonitrile precipitation,ethyl acetate extraction and DMF / acetonitrile precipitation. The results showed that when DMF / acetonitrile precipitation method was used,the concentration of diclazuril in rabbit plasma had a good linear relation with the response value,and the extraction recovery rate was over 85%. The method was suitable for diclazuril extraction in rabbit plasma.

关 键 词：家兔 血浆 地克珠利 提取方法 

分 类 号：S829.1[农业科学—畜牧学]