喘可治治疗肾阳虚型少精症实验研究  

Experimental Study of Chuankezhi on Oligospermia in Sprague-Dawley Rats with Kidney-Yang Deficiency

作　　者：符卓韬[1] 孟聪[2] 王志刚[1] 丘勇超[1] 

机构地区：[1]广州中医药大学第一附属医院泌尿外科,广东广州510405 [2]广州市中医医院妇科,广东广州510140

出　　处：《新中医》2016年第2期243-246,共4页New Chinese Medicine

摘　　要：目的:观察喘可治注射液对肾阳虚型少精症雄性大鼠的精子数量及致雌性大鼠受孕情况的影响。方法:将24只造模成功的肾阳虚雄性大鼠随机分为空白对照组、喘可治低剂量组[2.5 m L/(kg·d)]、喘可治高剂量组[10 m L/(kg·d)];每天上午9:00左右大鼠行游泳实验;下午3:00左右对照组大鼠腹腔内注射生理盐水2.5 m L/(kg·d);喘可治低剂量组大鼠腹腔内注射喘可治注射液2.5 m L/(kg·d);喘可治高剂量组腹腔内注射喘可治注射液10 m L/(kg·d);晚上8:00左右雄性大鼠与雌性大鼠按1∶2同笼,第2天上午8:00观察雌性大鼠阴道涂片。连续7天。同时观察各组雄鼠的精子密度和睾丸组织学。结果:致雌鼠受孕数:对照组(0.63±0.71),喘可治低剂量组(1.11±0.93),喘可治高剂量组(1.67±0.97),喘可治高剂量组、低剂量组雌鼠怀孕数均高于对照组,差异均有统计学意义(P<0.05);喘可治高剂量组怀孕数高于低剂量组,但组间比较,差异无统计学意义(P>0.05)。精子密度与造模前比较:对照组明显减少,喘可治低剂量组略有减少,喘可治高剂量组无明显改变;睾丸组织学评价:对照组曲精细管较空虚,其内生精细胞排列不均,生精细胞明显减少,部分缺失,精子细胞数显著减少,有的甚至出现钙盐沉积;而喘可治低、高剂量组均可见精子细胞密集,且呈剂量依赖关系。结论:喘可治注射液对肾阳虚型少精症大鼠具有较好的治疗效果,可以改善睾丸功能,提高受孕率,且呈量效关系。Objective. To investigate the effect of Chuankezhi injection on the number of sperm in oligospermic male SD rats with Kidney-Yang deficiency and on fertility of these oligospermic rats. Methods： Twenty-four SD rats with Kidney-Yang deficiency were randomly divided into blank control group, Chuankezhi low-dose group[2.5mL/（kg.d）], Chuankezhi high-dose group [10 mL/（kg.d）]. SD rats were in the swimming test at about 9：00 am every morning. 2.5 mL/（kg.d） normal saline was intraperitoneally injected in the rats of the blank control group at about 3：00 pro, and 2.5 mL/（kg .d） Chuankezhi was intraperitoneally injected in the Chuankezhi low-dose group, 10 mL/（kg-d） in the Chuankezhi high-dose group. Then the male rats caged with female rats（1 ： 2 per cage） at about 8：00 pro. Vaginal smears in female rats were monitored at 8：00 am the next day. Treatment lasted for 7 days. Sperm density as well as testis histology in male rats were investigated. Results： The numbers of pregnant female rats caused by each group of male rats were 0.63 ± 0.71 in the blank control group, and 1.11 ± 0.93 in Chuankezhi low-dose group and 1.67 ± 0.97 in Chuankezhi high-dose group. The numbers of pregnant female rats in low-dose group and high-dose group were higher than those in the blank control group, the difference being significant（P 〈 0.05）. The numbers of pregnant female rats in high-dose group were higher than those in low-close group, but there being no significant difference（P 〉 0.05）. Sperm density of male rat was obviously low in the blank control group, slightly decreased in low-dose group, and no significant different in high-dose group. Testis of male rat in the blank control group exhibited as relatively empty seminiferous tubules, in which were sparse and unevenly arranged spermatogenic ceils, partially missing, with very small number of sperms. Calcium depositions were observed on some of the sperms. But dense sperm cells were observed in the testis of rat of high or low dose

关 键 词：喘可治 肾阳虚型 少精症 睾丸生精功能 怀孕 动物实验 大鼠 

分 类 号：R285.5[医药卫生—中药学]