miR-199a-3p/Nme2在TGF-β1信号通路中的作用分析  

Role of miR-199a-3p/Nme2 in TGF-β1 Signaling Pathway

作　　者：刘晓雯[1] 吴道兵[1] 刘智勇[1] 周世华[1] 段红艳[1] 李丹[1] 

机构地区：[1]湖南大学生物学院,湖南长沙410082

出　　处：《激光生物学报》2016年第1期46-51,共6页Acta Laser Biology Sinica

基　　金：国家自然科学基金资助项目(81270735;81571494)

摘　　要：通过microRNA芯片技术在小鼠GC-1 spg细胞中筛选发现microRNA-199a-3p(miR-199a-3p)受转化生长因子TGF-β1调节。为了进一步探讨二者的关系,通过基因克隆与载体构建技术、双荧光素酶报告基因检测及定量PCR实验,发现miR-199a-3p靶向识别肿瘤转移抑制基因2(Nme2)的3'非编码区(UTR)序列,且正向调控Nme2的表达。利用TGF-β1处理GC-1 spg细胞后,结果显示Nme2和miR-199a-3p在mRNA水平的表达均显著上调;进一步将miR-199a-3p和TGF-β1双重作用于GC-1 spg细胞后,结果表明Nme2的表达会明显增强,而且在TGF-β1通路中,miR-199a-3p被抑制的部分功能可能会被Nme2补偿。综上,miR-199a-3p对Nme2基因具有直接靶向识别和调控作用,且在参与TGF-β1信号通路的生物学效应中,二者在功能上相互关联。microRNA-199a-3p( miR-199a-3p) was screened to be regulated by transforming growth factor-β1( TGF-β1) in mouse GC-1 spg cells in our previous work. By gene cloning and vector construction,dual luciferase reporter gene detection and quantitative PCR experiments,we found that miR-199a-3p target to 3-' UTR sequence of Nme2 gene.And miR-199a-3p could obviously up-regulate expression of Nme2 mRNA. After treating GC-1 spg cells with TGF-β1,the results showed that the expression of mRNA level of Nme2 and miR-199a-3p were up-regulated. We also found out that the expression of Nme2 increased significantly after GC-1 spg teing treated by both miR-199a-3p and TGF-β1. And some functions of the miR-199a-3p may be compensated by Nme2 in TGF-β1 signaling pathway. In conclusion,we verified that Nme2 is the direct target gene of miR-199a-3p. The miR-199a-3p and Nme2 have some functional correlation in TGF-β1 signaling pathway.

关 键 词：miR-199a-3p Nme2 TGF-β1信号通路 

分 类 号：Q752[生物学—分子生物学]