血清不同温育条件对高密度脂蛋白胆固醇的稳定性影响研究  

Stability of High-Density Lipoprotein Cholesterol During Serum Incubation

作　　者：张丽娇[1] 王思明[1] 曾洁[2] 杨睿悦[1] 李红霞[1] 王抒[1] 董军[1] 陈文祥[1,2] 

机构地区：[1]卫生部北京医院/卫生部北京老年医学研究所卫生部老年医学重点实验室,北京100730 [2]卫生部临床检验中心,北京100730

出　　处：《现代检验医学杂志》2016年第1期21-24,共4页Journal of Modern Laboratory Medicine

基　　金：国家自然科学基金资助项目（81472035,81171647）.

摘　　要：目的探讨血清高密度脂蛋白胆固醇（HDL—C）在不同温度、时间下的储存稳定性。方法2015年5月自卫生部北京医院征集健康志愿者10人（男性4人，女性6人，年龄24～59岁），将收集的血清在4℃温育24h，25℃温育0，1，8和24h[分别含和不含卵磷脂胆固醇酰基转移酶（LCAT）抑制剂]，HPLC法测定血清总胆固醇（TC），总游离胆固醇（TFC），HDL—C以及HDL游离胆固醇（HDL—FC）。使用微软EXCEL软件进行结果分析。结果血清在4℃温育24h，造成HDL—FC和HDL—C变化（平均为-6．91％和-2．17％）；25℃温育24h造成TFC，HDL—FC及HDL广C的变化（平均为-13．70％，-25．88％和-1．53％）；25℃下LCAT抑制剂完全抑制TFC的降低、部分抑制HDL—FC的降低，使HDL-C降低幅度更大。结论血清贮存可造成胆固醇变化，这种变化取决于LCAT，胆固醇酯转移蛋白（CETP）的活性以及FC在脂蛋白之间的转移。因此，为提高血脂测定结果的准确性，应尽量减少不必要的血清贮存。Objective To examine the stability of high-density lipoprotein cholesterol （HDL-C） during serum incubation at different temperature and time periods. Methods Ten healthy volunteers （4 males and 6 females,aged 24 to 59 years） from Beijing Hospital were recruited in May 2015. Fasting venous blood samples were collected and centrifuged to separate the sera. Serum samples were incubated at 4℃ for 24 h,25℃ for 0,1,8 and 24 h （with or without an LCAT inhibitor）. Serum total cholesterol （TC）,total free cholesterol （TFC） HDL-C and HDL-FC were measured by the HPLC Method. Results HDL-FC and HDL-C changed -6.91% and -2.17% during serum incubation at 4℃ for 24h. TFC, HDL-FC and HDL-C changed significantly （averaged -13.70%,-25.88% and -1.53% respectively） during serum incubation at 25℃ for 24 h, in which the decrease of TFC and HDL-FC were inhibited by the addition of the LCAT inhibitor. The decrease of HDL-C was even higher in the presence of the LCAT inhibitor. Conclusion Serum TFC, HDL-C and HDL-FC levels changed during serum incubations, which were caused by the LCAT and CETP activities and the transfer of cholesterol among lipoproteins. For accurate measurement of serum HDL-C,prolonged serum storage should be avoided in clinical laboratories.

关 键 词：高密度脂蛋白 胆固醇 稳定性 高效液相色谱 血清温育 

分 类 号：R446.112[医药卫生—诊断学]