哮喘患者外周血单个核细胞TIM-3及Galectin-9的表达与临床意义  

Express of TIM-3 and Galectin-9 Genes in Peripheral Blood Mononuclear Cells from Patients with Asthma

作　　者：梁涛[1] 张杨[1] 许依婷 王宁[1] 蔡鹏程[1] 王晓蓓[1] 胡丽华[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院检验科,武汉430022

出　　处：《现代检验医学杂志》2016年第1期52-54,共3页Journal of Modern Laboratory Medicine

基　　金：武汉市科技攻关计划项目,项目编号：2013060602010235.

摘　　要：目的探讨T细胞免疫球蛋白黏蛋白3（TIM-3）和半乳糖凝集素-9（Galectin-9）基因在急性发作期哮喘患者外周血单个核细胞的表达及临床意义。方法收集2014年1月～10月门诊急性发作期哮喘患者60例（排除不符合者15例，确定入选45例）及30例健康体检者（对照组），以实时定量RT-PCR法分别对哮喘患者及体检者外周血单个核细胞（PB—MCs）中的TIM-3，Galectin-9和IFN-γ的表达情况进行同步测定，初步探讨TIM-3，Galectin-9在哮喘发病中的潜在作用。结果急性发作期哮喘患者PBMCs中TIM-3，Galectin-9及IFN-γ mRNA水平均分别高于健康对照组（U=458．5，P=0．019；U=437．5，P=0．010；U=260，P〈0．001），二者间的差异均有统计学意义。结论TIM-3／Galectin-9可能参与了哮喘的发生、发展，TIM-3或（和）Galectin-9有可能为治疗哮喘提供一个新的靶点。Objective To investigate the levels of the mRNA expression of TIM-3 and Galectin-9 in peripheral blood monocytes （PBMCs） of acute exacerbation asthma patients and their clinical significances. Methods 60 patients with acute exacerbation asthma （eliminating 15 cases of non-conform to the regulations） and 30 cases of healthy subjects were collected from January to October of 2014. Used fluorescence quantitative real-time reverse transcription-polymerase chain reaction to measure the mRNA expression of TIM-3 and Galectin-9 in PBMCs of patients with asthma and healthy controls. Results The expression of TIM 3 ,Galectin-9 and IFN-γ mRNA in the PBMCs from acute exacerbation asthma patients were all abnormally higher than healthy controls （U= 458.5, P = 0.019 ; U= 437.5, P= 0.010;U= 260, P〈0. 001）. There were statistically significant differences between them. Conclusion TIM-3/Galectin-9 pathway may participate in the occurrence,development of asthma. TIM-3 or （and） Galeetin-9 may prove to be an important target for treatments to asthma.

关 键 词：哮喘 T细胞免疫球蛋白黏蛋白3 半乳糖凝集素-9 

分 类 号：R562.25[医药卫生—呼吸系统] R392.11[医药卫生—内科学]