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作 者:梁涛[1] 张杨[1] 许依婷 王宁[1] 蔡鹏程[1] 王晓蓓[1] 胡丽华[1]
机构地区:[1]华中科技大学同济医学院附属协和医院检验科,武汉430022
出 处:《现代检验医学杂志》2016年第1期52-54,共3页Journal of Modern Laboratory Medicine
基 金:武汉市科技攻关计划项目,项目编号:2013060602010235.
摘 要:目的探讨T细胞免疫球蛋白黏蛋白3(TIM-3)和半乳糖凝集素-9(Galectin-9)基因在急性发作期哮喘患者外周血单个核细胞的表达及临床意义。方法收集2014年1月~10月门诊急性发作期哮喘患者60例(排除不符合者15例,确定入选45例)及30例健康体检者(对照组),以实时定量RT-PCR法分别对哮喘患者及体检者外周血单个核细胞(PB—MCs)中的TIM-3,Galectin-9和IFN-γ的表达情况进行同步测定,初步探讨TIM-3,Galectin-9在哮喘发病中的潜在作用。结果急性发作期哮喘患者PBMCs中TIM-3,Galectin-9及IFN-γ mRNA水平均分别高于健康对照组(U=458.5,P=0.019;U=437.5,P=0.010;U=260,P〈0.001),二者间的差异均有统计学意义。结论TIM-3/Galectin-9可能参与了哮喘的发生、发展,TIM-3或(和)Galectin-9有可能为治疗哮喘提供一个新的靶点。Objective To investigate the levels of the mRNA expression of TIM-3 and Galectin-9 in peripheral blood monocytes (PBMCs) of acute exacerbation asthma patients and their clinical significances. Methods 60 patients with acute exacerbation asthma (eliminating 15 cases of non-conform to the regulations) and 30 cases of healthy subjects were collected from January to October of 2014. Used fluorescence quantitative real-time reverse transcription-polymerase chain reaction to measure the mRNA expression of TIM-3 and Galectin-9 in PBMCs of patients with asthma and healthy controls. Results The expression of TIM 3 ,Galectin-9 and IFN-γ mRNA in the PBMCs from acute exacerbation asthma patients were all abnormally higher than healthy controls (U= 458.5, P = 0.019 ; U= 437.5, P= 0.010;U= 260, P〈0. 001). There were statistically significant differences between them. Conclusion TIM-3/Galectin-9 pathway may participate in the occurrence,development of asthma. TIM-3 or (and) Galeetin-9 may prove to be an important target for treatments to asthma.
关 键 词:哮喘 T细胞免疫球蛋白黏蛋白3 半乳糖凝集素-9
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