AST试剂中NADH含量变化对线性范围影响的研究  

Influence of NADH Concetration in the AST Reagent on Assay Performance

作　　者：穆润清[1] 王银铃[1] 颜青[1] 

机构地区：[1]中国医科大学附属第一医院检验科,沈阳110001

出　　处：《现代检验医学杂志》2016年第1期121-124,共4页Journal of Modern Laboratory Medicine

摘　　要：目的评估天门冬氨酸氨基转移酶（AST）试剂中还原型辅酶（NADH）含量变化对试剂盒线性范围的影响。方法对两个具有不同NADH含量的同一品牌AST试荆的线性范围、精密度、灵敏度、试剂空白等性能指标分别进行验证，利用己糖激酶法测定葡萄糖浓度的方法测定NADH的摩尔消光系数，进而评估NADH浓度变化对分析性能的影响。结果较高及较低NADH浓度AST试剂的R1试剂中NADH浓度分别为0．21mmol／L（基础吸光度为1.1876）和0．13mmol／L（基础吸光度为0．7567），NADH浓度降低可造成试剂盒的线性范围减小，检测上限由1629U／L降至1263U／L。NADH浓度变化对两种试剂的精密度，灵敏度，试剂空白等性能指标无明显影响。结论AST试剂盒的R1试剂中NADH浓度降低（基础吸光度减小）可造成样品检测线性范围减小，因此实验室通过合适的线性评估方案定义其AsT试刺能够满足线性范围的基础吸光度标准，对于判断试荆质量、保证尚值结果准确是很重要的。Objective To estimate the assay performance of two different kind of reagent with different concentration of NADH, Methods Verifieated function index of these two reagents,such as accuracy,sensitiveness,blank space and so on, arid determined the molar extinction coefficient of NADH using hexokinase method and calculated the NADH density in two kind of AST reagents, Then estimated the effect of concentation of NADH on the analysis performance. Results NADH concertration was 0.21 mmol/L,and the sample detection limit of the linear was 1 629 U/L. NADH concentration was 0.13 minol/L,and the sample detection limit of the linear was 1 263 U/L. So the gap between the two reagent＇s detection linear was visible. But there were no significant changes in other performance indicators such as detection sensitivity, reagent blank,precision. Conclusion The NADH concentration in the reagent of AST had greatimpact on the detecting linear range, so should pay attention to the potential change of linear range in the daily work.

关 键 词：还原型辅酶 天门冬氨酸氨基转移酶 线性范围 

分 类 号：R446.112[医药卫生—诊断学]