HPLC同时测定黄花列当中3个苯乙醇苷的含量  

HPLC simultaneous determination of three phenylethanoid glycosides in Orobanche pycnostachya

作　　者：李彩峰[1] 温爱平[1] 王晓琴[1] 韩国庆[1] 

机构地区：[1]内蒙古医科大学药学院,呼和浩特010110

出　　处：《药物分析杂志》2016年第2期291-295,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金(81260615);内蒙古医科大学英才培育项目(2015YCPY027)

摘　　要：目的：建立HPLC法同时测定黄花列当中3个苯乙醇苷类化合物（毛蕊花糖苷、crenatoside、2’-乙酰毛蕊花糖苷）的含量。方法：采用Inertsil C18（4.6 mm×250 mm,5.0μm）色谱柱,乙腈-0.l%磷酸水溶液（22∶78）为流动相,流速1.0 mL·min^-1,柱温25℃,检测波长330 nm。结果：毛蕊花糖苷、crenatoside和2’-乙酰毛蕊花糖苷在30 min内得到良好的分离,且进样量分别在0.104～5.20μg（r=0.999 8）、0.106～2.65μg（r=1.000 0）、0.058～1.15μg（r=1.000 0）范围内呈现良好线性;平均回收率（n=3）在96.24%～102.7%之间。13批黄花列当样品中毛蕊花糖苷、crenatoside、2’-乙酰毛蕊花糖苷3种成分的含量范围分别为1.40%～11.53%、1.35%～3.22%和0.15%～1.63%,其中含量高的2个成分是毛蕊花糖苷和crenatoside;不同产地的药材成分含量差异较大。结论：本方法操作简便,准确、重现性良好,可用于黄花列当药材和饮片的质量检测。Objective： To develop an HPLC method for simultaneous determination of three phenylethanoid glycosides （ acteoside, crenatoside, 2＇ -aeetylacteoside ） in Orobanche pycnostachya. Methods： HPLC was performed on an Inertsil C18 analytical column （4.6 mm × 250 mm, 5.0 μ m ）with aeetonitrile-0.1% aqueous phosphoric acid （ 22 ： 78 ） as the mobile phase at a flow rate of 1.0 mL· min^-1. The column temperature was 25 ℃, and the detection wavelength was set at 330 nm. Results： There was good linearity in the range of 0.104-5.20 μg （ r=0.999 8 ） for acteoside, 0.106-2.65 μg （ r=1.000 0 ） for crenatoside, 0.058-1.15 μg （ r=1.000 0 ） for 2＇ -acetylaeteoside, respectively. The average recoveries （ n=3 ） of aeteoside, crenatoside and 2＇ - aeetylacteoside were between 96.24%-102.7%. There were significant differences of the contents of three constituents in Orobanche pycnostachya from different sources. The content of acteoside is the highest, followed by crenatoside. The contents of acteoside, crenatoside and 2＇ -acetylacteoside in 13 batches of samples were1.40%-11.53%,1.35%-3.22% and 0.15%-1.63%, respectively. Conclusion： This method is proved to be simple, accurate and reliable, which can be used for quality inspection of Orobanche pycnostachya herbs and its decoction pieces.

关 键 词：黄花列当 独根草 毛蕊花糖苷 crenatoside 2’-乙酰毛蕊花糖苷 高效液相色谱法 不同产地样品比较 

分 类 号：R917[医药卫生—药物分析学]