基于全时段多波长融合指纹图谱的中药木蝴蝶中总黄酮类成分研究  

Fingerprint of Semen Oroxyli based on the full-time multi-wavelength fusion method

作　　者：李楠楠[1] 王帅[1,2,3] 孟宪生[1,2,3] 包永睿[1,2,3] 李天骄[1,2,3] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]辽宁省组分中药工程技术研究中心,辽宁大连116600 [3]辽宁省现代中药研究工程实验室,辽宁大连116600

出　　处：《中南药学》2016年第1期1-4,共4页Central South Pharmacy

基　　金："十二五"重大新药创制科技重大专项(No.20132X09507005-004)

摘　　要：目的利用自主研发的全时段多波长融合软件,建立中药木蝴蝶有效组分的指纹图谱。方法采用高效液相色谱法,Eclipse Plus C_(18)色谱柱(2.1 mm×100 mm,1.8μm),0.1%甲酸水溶液(A),流动相为乙腈(B),进行梯度洗脱(0~10 min,15%~57%B;10~15 min,57%~65%B),柱温:30℃,检测波长:256、280、300、320 nm,流速为0.4 m L·min^(-1)。结果不同产地的中药木蝴蝶指纹图谱与参照图谱相比相似度均>0.9,但是各产地有一定的差异性,根据聚类分析大体可分为3类,其中大理、广西、亳州、四川为一类,昆明、贵州为一类,丽江、湖南为一类。结论建立了中药木蝴蝶有效组分全时段多波长融合指纹图谱,经方法学考察,该方法简便、准确、重复性好,可以为中药木蝴蝶的质量控制提供依据。Objective To establish a fingerprint of Semen Oroxyli with the laboratory full-time multi-wavelength fusion software. Methods HPLC method was used. Eclipse Plus C18 column （2.1 mm×100 mm,1.8μm） was used, the mobile phase was 0.1% methanoic acid in water （A） and acetonitrile （B） with gradient elution （0 - 10 min, 15% - 57% B; 10 - 15 min, 57% - 65% B）. The detection wavelengths were 256, 280, 300 and 320 nm. The flow rate of the mobile phase was 0.4 mL · min- 1 and the temperature was 30℃ , Results The fin- gerprint of different provenances of Semen Oroxyli compared with the reference fingerprint, the similarity was more than 0.9, but the origin had certain differences. According to the cluster analysis, the drug can be roughly divided into 3 classes, Dali, Guangxi, Bozhou, Sichuan for the same kind; Kuuming, Guizhou for the same kind; Lijiang, Hunan for the same kind. Conclusion The method is simple, accurate and highly reproducible, and can provide basis for the quality control of Semen Oroxyli.

关 键 词：木蝴蝶 指纹图谱 全时段多波长融合 总黄酮类 

分 类 号：R286[医药卫生—中药学]