离子液体对微藻的急性毒性效应  

On the acute toxic effects of the ionic liquids on the microalgae

作　　者：吴洁[1,2] 陈晓娣[1,2] 韩萍芳[1] BEADHAM Ian 胡兰[2] 邓芸[2,4] 顾军[2,5] 

机构地区：[1]南京工业大学生物与制药工程学院,南京210009 [2]南京大学昆山创新研究院,江苏昆山215347 [3]School of Life Sciences, Pharmacy & Chemistry, Kingston University, Kingston KT1 2EE Surrey, UK [4]江南大学环境与土木工程学院,江苏无锡214122 [5]南京大学物理学院,南京210093

出　　处：《安全与环境学报》2016年第1期381-386,共6页Journal of Safety and Environment

基　　金：江苏省自然科学基金项目(BK2012218);昆山市可持续发展实验区建设科技专项(KS1204)

摘　　要：采用流式细胞术研究离子液体1-丁基-3-甲基咪唑氯盐（[C4mim][Cl]）和1-丁基-3-甲基咪唑四氟硼酸盐（[C4mim][BF4]）对四尾栅藻的毒性影响,测定了细胞增殖、叶绿素荧光、细胞膜完整性和酯酶活性的变化。结果表明,2种离子液体均可抑制细胞增殖,且抑制作用随离子液体质量浓度和暴露时间增加而增强。计算得到96 h[C4mim][Cl]和[C4mim][BF4]的半数效应质量浓度EC50分别为49.61 mg/L和42.22 mg/L。测试期间发现,[C4mim][Cl]（质量浓度20~200 mg/L）和[C4mim][BF4]（质量浓度20~150 mg/L）对叶绿素荧光强度有一定的抑制作用,对细胞膜完整性无明显影响,随离子液体处理时间延长,对酯酶活性表现为先刺激后抑制作用;经200 mg/L[C4mim][BF4]处理藻细胞96 h后,藻细胞叶绿素的平均荧光强度仅为对照组的20%,酯酶活性为对照组的5%,且95%细胞膜发生破裂。2种离子液体对四尾栅藻均有毒性作用,这表明离子液体一旦进入水环境,将会对水生环境带来一定风险。This paper takes it as a goal to identify and determinate the toxic effects of two kinds of ionic liquids （ILs） [ C4mim] [ C1] and [C4mim][BF4] ） on the green microalgae Scenedesmus quadricauda, a key indicator of the aquatic environmental stress. For this purpose, we have made an investigation of the cell proliferation, the chloro- phyll fluorescence, the membrane integrity and the esterase activity of Scenedesmus quadricauda via the flow cytometer （FCM） . In our ex- perimental tests, S. quadricauda was let to be exposed to ILs in a range of mass concentrations from 0, 20 mg/L 50 mg/L, 100 mg/L, 150 mg/L to 200 mg/L. When the tests were over, all the samples were subject to an analysis in an interval of time period of O, 24 h, 48 h, 72 h and 96 h. Here, both the ILs should confine the cell proliferation of S. quadricauda, with the EC50 values of 49.61 mg/L and 42.22 mg/L for [C4mim] [C1] and [C4mim] [BF4] after 96 h exposure, respectively. In addition, all the inhibition rate for the cell proliferation should be made increasing with the increase of both the ILs concentration and incubation time, though the concentra- tion dependence on the chlorophyll fluorescence proves to be less ob- vious. After being exposed to [ C4mim] [C1] with mass concentration rangefrom 20 mg/L to 200 mg/L and [C4mim] [BF4] with mass concentration range from 20 mg/L to 150 mg/L for 96 h, the average chlorophyll fluorescence intensity is 34%- 39% and 28%- 41% lower than that of the control group, respectively; While after being exposed to 200 mg/L [C4mim][BF4] for 96 h, the average chloro- phyll fluorescence intensity is just 20 % as against the control group. After being exposed to 200 mg/L [C4mim] [BF4] for 96 h, the cell membranes of 95 % of the cells tend to get damaged with all the other samples being able to keep their membrane integrity. For the samples exposed to [ C4mim] [ C1] at all concentrations, or [ C4mim] [ BF, ] at 20 - 150 mg/L, the esterase activity are likely to get stimulated l h later, and, then, in subsequ

关 键 词：环境学 离子液体 毒性 流式细胞术 叶绿素荧光 细胞膜完整性 酯酶活性 

分 类 号：X506[环境科学与工程—环境工程] Q93[生物学—微生物学]