四溴双酚A对斑马鱼游动行为的毒性效应研究  

Toxic impact of tetrabromobisphenol A( TBBPA) on the swimming behavior of zebra fish via a biological observation and monitoring

作　　者：周斯芸[1] 张瑛[1] 魏倡[1] 倪芳 全燮[1] 

机构地区：[1]大连理工大学环境学院,工业生态与环境工程实验室,辽宁大连116024

出　　处：《安全与环境学报》2016年第1期387-391,共5页Journal of Safety and Environment

基　　金：国家自然科学基金重大国际合作研究项目(NSFC-JST,21261140334);国家科技重大专项水体污染控制与治理项目(2012ZX07202-006)

摘　　要：通过3D生物传感器实时监测暴露在四溴双酚A（TBBPA）污染水体中的斑马鱼游动行为的变化,分析TBBPA对斑马鱼的毒性效应。3D生物传感器利用两个垂直摄像头来监测鱼的游动行为,包括游速和垂直位置等。结果表明,不同质量浓度区间的TBBPA对斑马鱼游动行为的影响有所差别：低质量浓度（〈2 mg/L）时对斑马鱼游动行为几乎没有影响;中质量浓度（5~10 mg/L）时,斑马鱼的游速先增大后减小,随TBBPA质量浓度增加游速增大幅度也有所增加,且游动位置上移;高质量浓度（100 mg/L）时,斑马鱼游速随TBBPA质量浓度增高而降低,且游动位置下移。这表明可以通过监测斑马鱼的行为变化来表征TBBPA对水生态的毒性效应,采用游速和游动位置作为判断鱼类行为异常的指标较之传统方法更能表征低浓度、低毒性的污染物毒性。The paper is inclined to make a biological observation and monitoring research in hoping to find the toxic impact of tetrabromo- bisphenol A （TBBPA） on the swimming behavior of zebra fish, which can be taken as a reactive and additive flame retardant in the trace concentration in the environment. As is known, TBBPA is an en- docrine disruptor and immuno-toxicant, for which its potential risk has been drawing more and more attention when it is discharged into the environmental water reservoir. In this paper, we have investigated the biotoxic effect of TBBPA on the swimming behavior of zebra fish （ Danio rerio ） via the real-time monitoring observation with 3D- biosensor, in which two cameras were set in a 3D-biosensor to make a follow-up study of their swimming behavior （i.c. swimming speed and vertical position）. The results show that the effects of TBBPA on the swimming behavior of zebra fish have been changing with the dif- ferent TBBPA concentrations. However, there exist no significant changes with the swimming behaviors when the fish were exposed to low concentrations （ 〈 2 rag/L） of TBBPA. As to the swimming speed of the said fish, it has been found increasing first and then dropping down bit by bit when the fish were exposed to the middle concentrations （3 mg/L to 5 rag/L）, with the fish trying to swim in the upper layers. More accurately speaking, when exposed to the high concentrations （10 mg/L to 100 rag/L）, the swimming speed of the said fish tend to decrease with the concentration increment of TBBPA and then they were to stay bottom-up. It suggests that the toxic effect of TBBPA can be characterized by the real time monitor- ing of the fish swimming behavior. When comparing the swimming behavior to the traditional toxicity index （e. g. lethality rate）, the swimming behavior （i. e. the swimming speed and the vertical posi- tion） of the fish can be felt in a more sensitive way even under the lower concentration of pollutants. This suggests that what we have done in moni

关 键 词：环境学 生物监测 四溴双酚A 斑马鱼 

分 类 号：X8[环境科学与工程—环境工程]