乙肝病毒慢性感染者HBV-DNA定量与血清学标志物及年龄性别关系  

The relationship Between the HBV DNA quantification and HBV marker,age and gender among chronic patients with hepatitis B virus infection

作　　者：王明儒[1] 刘胜军 刘中芹 王晓琼[1] 刘召红[1] 崔佳[1] 王颖[1] 

机构地区：[1]寿光市疾病预防控制中心,山东寿光262700 [2]寿光市古城医院,山东寿光262718

出　　处：《预防医学论坛》2016年第1期1-3,共3页Preventive Medicine Tribune

基　　金：国家科技重大专项艾滋病和病毒性肝炎等重大传染病防治专项"山东省乙型病毒性肝炎防治综合示范区规模化现场流行病学和干预研究"(2013ZX10004902)

摘　　要：目的探讨乙肝病毒慢性感染者HBV-DNA定量与血清学标志物(Hepatitis B virus serologic marker,HBVm)及年龄、性别关系。方法 2013年9~12月对1 506例HBsAg阳性的乙肝病毒慢性感染者血清用实时荧光定量(PCR)测定HBV-DNA含量,用酶联免疫吸附试验(ELISA)测定HBVm,用速率法检测ALT、AST。结果 HBV-DNA总检出率为48.48%(730/1 506),均值为6.58×107拷贝/ml,HBeAg阳性组检出率83.52%(228/273),平均拷贝数1.78×108;HBeAg阴性组检出率40.71%(502/1 233),平均拷贝数1.15×107。HBV-DNA检出率和平均拷贝数HBeAg阳性组均高于阴性组(P<0.01)。男性HBV-DNA检出率53.22%(438/823)高于女性42.75%(292/683)(P<0.01)。HBVDNA阳性组ALT、AST>40u/L的异常率分别是39.53%(289/731)、64.98%(475/731),均值分别是55.38、46.55u/L;HBV-DNA阴性组ALT、AST>40u/L的异常率分别是12.65%(98/775)、12.26%(95/775),均值分别是25.39、28.85u/L。ALT、AST异常率和均值HBV-DNA阳性组均高于阴性组,差异有统计学意义(P<0.01)。结论在各种HBVm模式中,以HBeAg阳性者的病毒复制水平最高。HBV-DNA检出率与HBVm模式、性别、年龄、以及ALT、AST异常率有关,平均拷贝数与性别、年龄无关。Objective To explore the relationship between the HBV-DNA quantification and HBV marker（Hepatitis B virus seroloqic marker） ,age and gender among chronic patients with hepatitis B virus infection. Methods During Sep- tember-December,2013, HBV-DNA in sera of 1 506 hepatitis B patients who had positive reaction to HBsAg test were de- termined using fluorescence quantitative polymerase chain reaction （FQ-PCR） and HBVm using enzyme-linked immu- nosorbent assay（ELISA）. ALT and AST were examined by means of speed methods. Results The total detection rate was 48.48% （730/1 506）, the mean value was 6.58 × 107 copies/ml. The positive rate of HBeAg was 83.52% （228/273）, the average copy was 1.78 × 10s copies/ml;the negative rate of HBeAg was 40. 71% （502/1 233）, the average copy was 1.15 X 107 copies/ml. The former was significantly higher than the latter（ P d0.01）. The detection rate of HBV-DNA in males（53. 220/00） and in females（42.75%） was significantly different（ P d0.01）. The abnormal liver function rate in the HBV-DNA positive group was 39.53% （289/731）（an ALT reading of greater than 40 u/L） and 64.98% （475/731）（an AST reading of greater than 40 u/L） ,which the mean of them were 55.38 u/L （ALT） and 46.55 u/L （AST）. While in the HBV-DNA negative group, the rate was 12.65 % （98/775）（an ALT reading of greater than 40 u/L） and 12.26 % （95/ 775） （an AST reading of greater than 40 u/L）,the mean was 25.39 u/L （ALT） and 28.85 u/L（AST）. And the abnormal rate and the mean of ALT and AST^40 u/L in positive patients were significantly higher than that in negative patients（ P〈0. 01）. Conclusion The virus replication of HBeAg-positive patients is the highest in various HBVm groups. There is obvious relationship between HBV-DNA detection rate and age,gender, HBVm groups as well as the abnormal rate of ALT,AST. However,the average copy number has nothing to do with age and gender.

关 键 词：乙型肝炎病毒 荧光定量PCR HBV-DNA 

分 类 号：R512.62[医药卫生—内科学]