血浆DNA诊断早期急性ST段抬高型心肌梗死的研究  

Research on cell-free DNA in the early diagnosis of acute myocardial infarction

作　　者：王敏[1,2] 郭延松[1] 

机构地区：[1]福建医科大学省立临床医学院,福建省立医院心内科,福州350001 [2]福建中医药大学第一临床医学院

出　　处：《福建医药杂志》2016年第1期1-4,共4页Fujian Medical Journal

基　　金：福建省卫生系统中青年骨干人才培养项目资助(2014-ZQN-ZD-2);福建省自然科学基金项目资助(2011J01131);福建省医学创新课题资助(2011-CXB-4);福建省临床重点专科建设项目(心血管内科)资助

摘　　要：目的探讨血浆DNA在急性ST段抬高型心肌梗死早期的变化,评估其在心肌梗死诊断方面的价值。方法应用实时荧光定量PCR法对28例急性ST段抬高型心肌梗死发生6h内血浆DNA浓度和肌钙蛋白I进行监测,并与50例健康对照组对比分析。结果急性心肌梗死胸痛发作6h内的28例患者,血浆DNA浓度经对数转换后的平均数值为4.16±0.25,健康对照组对数转换后平均数值为2.39±0.12,急性心肌梗死组高于健康对照组。血浆DNA相关的ROC分析示曲线下面积为0.870,95%置信区间为(0.764,0.976)。结论血浆DNA可作为急性ST段抬高型心肌梗死早期诊断的新标志物。Objective To investigate concentration of cell-free DNA changes following an hyperacute ST-elevation myo- cardial infarction （ASTEMI） and to assess any correlations With ASTEMI. Methods Serial cell-free DNA level were determined by quantitative Real-t/me PCR in 28 ASTEMI patients and in 50 healthy individuals. Concentrations of cell-free DNA in patients were compared with healthy subject. At the same time, cTnI was determined. And receiver operating characteristic （ROC） curves were used to analyse diagnosis value of cell-free DNA in acute myocardial infarction. Results Cell-free DNA concentra- tions in patients with ASTEMI showed a significant elevation compared with healthy controls ELg （mean＋ SD） ： （4.16±0. 25） vs （2.39±0.12）, P〈0.05）]. ROC curve indicated that area under the curve was 0. 870 （P〈0, 05） and 95% confidence inter- val ranged 0. 764 to 0. 976. Conclusion Cell-free DNA may be a valuable marker for early diagnosing in ASTEMI.

关 键 词：急性心肌梗死 血浆DNA 标志物 诊断 

分 类 号：R542.22[医药卫生—心血管疾病]