Fc受体结合型与非Fc受体结合型CD3单抗的制备  

Purification of Fc receptor binding and Fc receptor non-binding anti-CD3 monoclonal antibody

作　　者：李晴[1] 陈剑晖[2] 李晓帆[2] 

机构地区：[1]福建省立医院妇产科,福州350001 [2]福建医科大学附属协和医院

出　　处：《福建医药杂志》2016年第1期46-49,共4页Fujian Medical Journal

基　　金：教育部高等学校博士学科点专项科研基金(20123518120006);福建省自然科学基金青年创新项目(2012J05148)

摘　　要：目的研究Fc受体结合型与非Fc受体结合型CD3单抗的制备及单抗的特性。方法利用杂交瘤细胞株扩增,使用体外培养法制备Fc受体结合型CD3单抗,利用动物体内诱生法制备非Fc受体结合型CD3单抗。使用亲和层析法纯化抗体并与市售的CD3单抗比较,行淋巴细胞培养、流式细胞术等了解Fc受体结合型与非Fc受体结合型CD3单抗的特性。结果使用体外培养法制备蛋白G琼脂糖纯化了Fc受体结合型CD3单抗;使用动物体内诱生法制备A蛋白亲和层析法纯化了非Fc受体结合型CD3单抗;CD3单抗与市售的CD3单抗一致;Fc受体结合型与非Fc受体结合型CD3单抗的增殖及T细胞受体内化效应和文献报道一致。结论利用杂交瘤细胞株扩增并成功制备了Fc受体结合型与非Fc受体结合型CD3单抗,并为后续工作的进行奠定了良好的基础。Objective To purify Fc receptor binding and Fc receptor non-binding anti-CD3 monoclonal antibody. Methods Hybridoma strains were amplified. In vitro culture was applied to produce Fc receptor binding anti-CD3. Ascites were applied to produce Fc receptor non-binding anti-CD3. Affinity chromatography was used for the purification of antibodies. The products were compared to the commercial CD3 monoclonal antibody. Also, lymphocyte culture and flow cytometry were used to under- stand the characteristics of Fc receptor binding and Fc receptor non-binding CD3 monoclonal antibody. Results Fc receptor binding anti-CD3 was produced by protein G-agarose purification. Fc receptor non-binding anti-CD3 was produced by Protein A ascites purification. The products were not different with the commercial CD3 monoclonal antibody and consist with previous pa- pers. Conclusion By purification of Fc receptor binding and Fc receptor non-binding anti-CD3 monoclonal antibody, a good foundation is established for the follow-up work.

关 键 词：Fc受体结合型 非Fc受体结合型 CD3单抗 T细胞受体 细胞因子风暴 

分 类 号：R392[医药卫生—免疫学]