健脾生血片益气生血的作用机制研究  

Study on Tonifying Qi and Producing Blood Mechanism of Jianpi Shengxue Tablets

作　　者：李春福[1] 张雪琼[1] 翟丽 邱彤[1] 黄志军 游东阁 郭小娟 熊富良[1] 赵刚 

机构地区：[1]武汉理工大学化学化工与生命科学学院,武汉4300701 [2]健民药业集团儿童药物研究院

出　　处：《中国药师》2016年第2期209-212,共4页China Pharmacist

基　　金：国家自然科学基金项目(编号:31300791)

摘　　要：目的:考察健脾生血片对低铁贫血模型大鼠的作用机制。方法:SD大鼠50只随机分为5组,分别为正常组、硫酸亚铁组、健脾生血片低、中、高剂量(0.65,1.3,2.6 g·kg^(-1))组,建立低铁贫血模型,健脾生血片各组及硫酸亚铁组水溶液灌胃,给药2周后,检测大鼠肠黏膜二价金属转运体(DMT-1)表达量及肝脏Hepcidin mRNA表达量。结果:健脾生血片中、高剂量组能显著提高DMT-1蛋白表达量,硫酸亚铁组DMT-1蛋白表达下调。肝脏中Hepcidin mRNA表达量随着给药剂量的增加而增加。结论:健脾生血片中药成分对上调DMT-1起到重要作用,通过改善Hepcidin mRNA的表达以达到补铁作用。Objective： To study the tonifying Qi and producing blood mechanism of Jianpi Shengxue tablets. Methods： SD rats were randomly divided into the normal group, FeSO4 group, and low, medium and high dosage groups （0.65,1.3,2.6 g · kg- 1 ）. All the groups except the normal group received low iron diet, to induce anemia. After the two-week intragastric administration, real-time PCR was carried out to identify the changes of Hepcidin mRNA expression, while western blot was applied to test the changes of DMT- 1 protein expression. Results： The medium and high dosage groups could significantly increase the DMT-1 expression compared with the normal group, while FeSO4 could reduced the DMT-1 expression. The Hepcidin expression in liver was increased along with the increase of the dosage. Conclusion： The Chinese medicine ingredients in Jianpi Shengxue tablets is beneficial to the expression of DMT- 1 protein and the expression of Hepcidin mRNA in liver, which can finally improve the iron supply in the body.

关 键 词：健脾生血片 二价金属转运体蛋白 HEPCIDIN基因 作用机制 低铁贫血模型 

分 类 号：R285.5[医药卫生—中药学]