Box-Behnken设计响应面法优选水杨梅总黄酮提取工艺研究  

Extraction Technology Optimization of Total Flavonoids in Geum Aleppicum by Box-Behnken Design and Response Surface Methodology

作　　者：陈程[1] 冯锁民[1] 罗国平[1] 张存劳 杜萌[1] 

机构地区：[1]西安医学院药学院,西安710021

出　　处：《中国药师》2016年第2期241-243,250,共4页China Pharmacist

基　　金：西安医学院青年科研基金项目(编号:2015QN07)

摘　　要：目的:采用Box-Behnken设计响应面法优化水杨梅的最佳提取工艺。方法:以水杨梅中总黄酮含量为评价指标,通过单因素试验分别考察乙醇浓度、液料比、提取时间三个因素对总黄酮含量的影响,采用Box-Behnken设计响应面法对水杨梅的提取工艺参数进行优化。结果:水杨梅的最佳提取工艺:乙醇浓度为50%,液料比为4.2倍,提取时间为80 min,在该条件下平均提取量12.590 0 mg·g^(-1)。结论:Box-Behnken设计响应面法优化水杨梅总黄酮提取工艺合理,建立的数学模型和试验数据相符,具有较好的预测性。Objective： To analyze and optimize the extraction technology of Geum aleppicum by Box-Behnken design and response surface methodology. Methods： With the content of total flavonoids as the index, the effects of three factors including the solvent-solid ratio, ethanol concentration and extraction time on the content of total flavonoids were studied by single factor experiment, and the extraction technology was optimized by Box-Behnken design and response surface methodology. Results： The optimal extraction conditions of Geum aleppicum were as follows：the solvent-solid ratio was 4.2, the ethanol concentration was 50% and the extraction time was 80 min. Under the above conditions, the average yield was 12. 590 0 mg · g-1. Conclusion： Optimizing extraction technology of the total flavonoids in Geum aleppicum by Box-Behnken design and response surface methodology is reasonable, and the mathematical model is consistent with the experimental data, which has good predictability.

关 键 词：水杨梅 Box-Behnke设计 总黄酮 提取 

分 类 号：R284.2[医药卫生—中药学]