四妙君逸软膏不同提取工艺的止痛作用比较研究  

Comparative Study on Analgesic Effect of Simiao Junyi Ointment with Different Extraction Process

作　　者：陈光宇[1] 陈善军[1] 韩军涛[1] 何永恒[2] 何群[1] 

机构地区：[1]湖南中医药大学药学院,长沙410004 [2]湖南中医药大学第二临床医学院

出　　处：《中国药师》2016年第2期270-273,共4页China Pharmacist

基　　金：湖南省长沙市科技局计划项目(编号:K1003068-31);湖南省科技厅计划项目(编号:2009SK3125);湖南省研究生精品课程建设项目(编号:KC2011B026)

摘　　要：目的:通过镇痛实验筛选四妙君逸软膏中4味药材的最佳提取工艺。方法:采用小鼠热板法及小鼠扭体法进行镇痛实验,观察四妙君逸软膏中4味药材不同提取工艺的镇痛药效。结果:由等级一致性检验结果可知,小鼠热板法各组数据无统计学差异,不同提取工艺制得的四妙君逸软膏药效基本一致;小鼠扭体法各组数据均有统计学差异,花椒、延胡索采用超临界CO_2萃取,没药用95%醇提,三七用50%醇提制成的四妙君逸软膏能很好地抑制小鼠醋酸引起的痛阈,镇痛作用最明显,故该提取工艺最优。结论:通过镇痛实验筛选得到的四妙君逸软膏提取工艺科学、合理、可行,适合现代化中药制剂的研究与开发。Objective： To screen the best extraction process of four medicinal materials in Simiao Junyi ointment by analgesic experiments. Methods： The analgesic experiments were performed by the hot-plate test and writhing test in mice to select the best extraction process of four medicinal materials in Simiao Junyi ointment. Results ： According to the results of level consistency check, the data of hot-plate test in mice in different groups had no significant difference, and the pesticide effect of Simiao Junyi ointment prepared by different extraction process was similar. However, the data of writhing test had significant difference. The extraction process of the ointment with the best analgesic activity was as follows： pepper and rhizoma corydalis were extracted by SFE-C02, myrrha and pseudo-ginseng was extracted by 95% and 50% ethanol, respectively. Conclusion： The optimal extraction process tested by analgesic experiments is scientific, reasonable and feasible, and suitable for the research and development of modern traditional Chinese medicine preparations.

关 键 词：四妙君逸软膏 提取工艺 镇痛作用 

分 类 号：R285.5[医药卫生—中药学]