石墨烯量子点荧光探针测定肾上腺色腙  

Detection of Carbazochrome by Graphene Quantum Dot Fluorescence Probe

作　　者：马红燕[1] 王艳妮[1] 

机构地区：[1]延安大学化学与化工学院陕西省反应工程重点实验室,陕西延安716000

出　　处：《发光学报》2016年第2期230-236,共7页Chinese Journal of Luminescence

基　　金：陕西省自然科学基础研究计划(2014JM2056);延安大学研究生科研创新项目资助

摘　　要：通过高温裂解柠檬酸合成了水溶性石墨烯量子点（GQDs）,并应用钝化剂PEG2000进行修饰,提高了GQDs的量子产率。应用荧光光谱、紫外-可见光谱、红外光谱对其发光特性进行了研究,测定了荧光寿命。实验发现,在p H=7.40的Tris-HCl缓冲液中,肾上腺色腙（CBZC）对GQDs荧光强度有明显的猝灭作用。基于此提出了以GQDs为探针测定肾上腺色腙的新方法。实验考察了缓冲溶液用量、缓冲溶液种类、量子点浓度、反应时间以及表面活性剂等多种因素对反应体系的影响。当量子点浓度为2.3×10-3mol/L时,肾上腺色腙浓度在4.0×10-7~1.2×10-5mol/L（0.995 6）范围内与荧光猝灭值ΔF呈良好的线性关系,方法检出限为1.5×10-7mol/L,相对标准偏差为0.15%（n=5,c=4.0×10-6mol/L）。该方法对于样品中肾上腺色腙含量测定的回收率为97.46%~101.6%。通过测定温度对猝灭常数的影响以及紫外-可见吸收光谱的变化确定了二者的猝灭过程和相互作用力类型。Water-soluble graphene quantum dots （GQDs） were synthesized by the pyrolysis of citric acid. Its fluorescent quantum yield was remarkly increased by PEG2000 modified. The lumines- cence characteristics of GQDs were studied by fluorescence spectroscopy, ultraviolet-visible spectroscopy, infrared spectroscopy as well as the fluorescence lifetime. A novel method for quantitative determination of trace carbazochrome （CBZC） was developed based on the fluorescence quenching of GQDs in pH 7.40 Tris-HC1 buffer solution. A wide range of reaction parameters, such as concentra- tion of GQDs, reaction time, surfactant, sorts and amount of buffer was examined. The screening resuits show that the AF was linearly related with the carbazochrome concentration from 4.0 × 10 -7 to 1.2 × 10-5 mol/L（0. 995 6） with the detection limit of 1.5 × 10 -7 mol/L. The relative standard deviation is 0.15% （n =5, c =4.0 × 10-6 mol/L）, when the concentration of GQDs is 2.3 × 10 -3 mol/L. The method has been used for the determination of carbazochrome in samples with the recoveries of 97.46% - 101.6%. The interaction mechanism and main sorts of binding force between GQDs and carbazochrome were investigated by the temperature effect on the quenching constants and UV-visible absorption spectrometry.

关 键 词：石墨烯量子点(GQDs) 荧光探针 肾上腺色腙(CBZC) 柠檬酸 

分 类 号：O657.3[理学—分析化学]