丹龙醒脑方对脑缺血再灌注大鼠血管新生及碱性成纤维细胞生长因子表达的影响  

Effects of Danlong Xingnao Decoction(丹龙醒脑方) on Angiogenesis and b FGF Protein Expression in Rats with Cerebral Ischemia-Reperfusion

作　　者：张秋雁[1] 徐瑾瑜 梁昊[1] 唐金强[1] 朱伟[1] 苏剑峰[1] 唐映红[1] 逯晶[1] 

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]华恩堂养生管理有限公司,湖南长沙410000

出　　处：《中医药导报》2016年第4期20-23,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖南省高校创新平台开放基金项目(12K090)

摘　　要：目的:研究丹龙醒脑方对脑缺血再灌注大鼠血管新生及碱性成纤维细胞生长因子(b FGF)的影响。方法:用线栓法制备大鼠大脑中动脉栓塞缺血再灌注(MCAO)模型,2 h后开放血流再灌注。大鼠造模成功后分为6组,丹龙醒脑方大(14.76 g/kg)、中(7.38 g/kg)、小(3.69 g/kg)剂量组,尼莫地平(10.80 mg/kg)组,模型组,假手术组,灌胃7 d后处死大鼠取脑,用免疫组化法测定缺血海马区的内皮细胞数和微血管密度(MVD)及bFGF的表达。结果:与假手术组比较,模型组大鼠内皮细胞计数及微血管密度、bFGF蛋白表达均增强(P<0.05或P<0.01);与模型组比较,丹龙醒脑方组内皮细胞数、微血管密度及bFGF蛋白表达均有明显升高,差异有统计学意义(P<0.05)。结论:丹龙醒脑方能够促进脑缺血再灌注大鼠缺血脑组织血管新生,对缺血脑组织具有保护作用,其作用机制可能与上调bFGF蛋白表达有关。Objective： To investigate the effect of Danlong Xingnao Decoction （DLXND, 丹龙醒脑方） on angiogenesis and bFGF protein expression in rats with cerebral ischemia-reperfusion. Methods： The MCAO rat model was created by modified Longa method, and blood reperfusion opened after 2 hours. The model rats were randomly divided into six groups, High-dose （14.76 g/kg）, moderate-dose （7.38 g/kg） and low-dose （3.69 g/kg） DLXND groups, nimodipine group （10.80 mg/kg）, model group and Sham group. The rats were given by garage once for 7 days. The microvessel vascular density （MVD） was observed and the expression of bFGF at protein level were studied in immuneohistochemical method. Results： Compared with the Sham group, the microvessel density and bFGF were higher significantly in model group （P〈0.05 or P〈0.01）. Compared with the model group, the levels of MVD and the protein expression of bFGF were significantly enhanced in DLXND groups （P〈0.05）. Conclusions： DLXND could promote angiogenesis and protect ischemic brain tissue in rats with cerebral ischemi- a-reperfusion, which mechanismsand may be related to upregulation of the bFGF expression.

关 键 词：脑缺血再灌注 大鼠 丹龙醒脑方 血管新生 BFGF 

分 类 号：R285.5[医药卫生—中药学]