2014-2015监测年度中国B型流感病毒病原学特征分析  

Virological characteristics of influenza B virus isolated in China's Mainland during 2014-2015 influenza surveillance year

作　　者：李希妍[1] 成艳辉[1] 谭敏菊[1] 隗合江[1] 蓝雨[1] 黄维娟[1] 肖宁[1] 王大燕[1] 舒跃龙[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所国家流感中心,卫生和计划生育委员会医学病毒和病毒病重点实验室,北京102206

出　　处：《中华微生物学和免疫学杂志》2016年第1期9-15,共7页Chinese Journal of Microbiology and Immunology

基　　金：基金项目：“十二五”重大传染病专项课题（2012ZX10004215-002）

摘　　要：目的分析2014--2015流感监测年度我国B型流感病毒的流行情况及与流感疫苗株的匹配性。方法选择本监测年度中国分离的B型流感病毒，进行抗原性分析并对全基因组进行测序，构建进化树，分析我国B型流感病毒的变异情况，并比较其与疫苗株的匹配性。结果本年度我国B型流感病毒活动水平较低，占所有收检病毒的29．94％，而B型流感病毒中Yamagata系毒株明显多于Victoria系。抗原性分析显示，Yamagata系流感病毒绝大部分为疫苗株B／Massachusetts／2／2012的类似株（95．1％），而Victoria系病毒只有48．3％为四价流感疫苗组分B／Brisbane／60／2008的类似株，但与我国Victoria系流行代表株B／北京海淀／1386／2013抗原类似的比例为100％。基因特性分析显示有19株Yamagata系毒株为BY—HA／BV—NA系问重配毒株。分析NA基因的耐药位点发现8株Yamagata系病毒有已知的敏感性降低的氨基酸位点突变。结论2014--2015流感监测年度我国B型Yamagata系病毒与疫苗株匹配较好，但Victoria系病毒与四价疫苗株匹配性不佳，因此应及时分析我国流行病毒的抗原性和基因特性，推选出与我国流行病毒相匹配的疫苗株。Objective To analyze the epidemiological characteristics of the influenza B virus strains circulating in China's Mainland during 2014-2015 influenza surveillance year and to study the genetic similarity between them and the vaccine strains. Methods Hemagglutinin inhibition test was performed to analyze the antigenicity of influenza B virus strains isolated in China's Mainland during 2014-2015 influenza surveillance year. The nucleotide sequences of those virus strains were determined by using Sanger dideoxy sequencing. The phylogenetic trees were constructed based on the genes encoding the hemagglutinin 1 （ HA1 ）. The genetic characteristics of the influenza B virus strains were determined by comparing with the current vaccine strains. Results The influenza B virus strains accounted for 29.94% of all influenza virus strains isolated during 2014 to 2015. The influenza B virus strains belonged to Yamagata lineage were more than that belonged to Victoria lineage. Most of the Yamagata lineage virus strains were antigenically similar to the B/Massachusetts/2/2012 vaccine strain （95.1% ）. Only 48.3% of the prevalent virus strains were antigenically similar to the quadrivalent vaccine strain B/Brisbane/60/2008-1ike virus, while 100% of the strains were antigenically similar to the representative circulating strain B/Beijing-Haidian/1386/2013 in China's Mainland. Phylogenetic analysis showed that 19 Yamagata lineage virus strains were BY-HA/BV-NA intra-lineage reassortants. Mutations associated with drug resistance in genes encoding the neuraminidase （NA） were found in 8 Yamagata lineage strains, resulting in reduced drug sensitivity. Conclusion The WHO recommended trivalent influenza vaccine could protect from Yamagata lineage influenza B virus infec- tion during 2014-2015 influenza surveillance year. However, the Victoria lineage influenza B virus strainswere not match with the quadrivalent influenza vaccine strain. This study indicated that the antigenic and ge- netic characteristics of the prevalent influ

关 键 词：B型流感病毒 抗原特性 基因特性 

分 类 号：R511.7[医药卫生—内科学] R446.6[医药卫生—临床医学]