在体多通道电生理记录技术在大鼠痛情绪研究中的应用  

The application of in vivo multi-channel recording methods in the studies of the affective pain in rats

作　　者：李振华[1] 杨洋[2] 侯苗苗 王媛[1] 秦霞[1] 张策[1] 张宇[1] 

机构地区：[1]山西医科大学生理学系,太原030001 [2]山西医科大学临床医学系,太原030001

出　　处：《中国应用生理学杂志》2016年第1期41-45,共5页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目(81371254)

摘　　要：目的:通过已建立的完全弗氏佐剂(CFA)诱导的条件位置逃避(CPA)行为模型,利用在体多通道电生理学记录技术,结合行为观察研究大鼠发生CPA反应时前扣带皮层(ACC)脑区神经元的电活动。方法:电极制作,电极埋置,在体多通道技术记录清醒大鼠r ACC脑区神经元的电活动及其CPA行为记录。结果:1自制的多通道阵列电极可成功记录到ACC神经元的放电活动;2大鼠脚掌注射CFA前后分别与不同环境匹配后,大鼠处于"痛环境"与"非痛环境"时ACC神经元spike的发放频率分别为痛环境(0.85±1.38)imp/s,非痛环境(0.22±0.97)imp/s(P<0.05,n=26);3行为学分析痛环境适应前(303.55±61.77)s对比痛环境适应后(140.32±33.52)s(P<0.05,n=6)。上述结果显示,脚掌注射CFA的大鼠处于痛环境时诱发ACC神经元spike放电频率增强与行为上的逃避反应同步发生;脚掌注射生理盐水的对照组并无上述反应趋势。结论:大鼠r ACC脑区神经元电活动与疼痛所致的痛厌恶相关情绪的发生有着密切的联系。Objective： To record the electrical activities of Antirior cingulate cortex（ACC）neurons by in vivo multi-channel recording methods using the model of complete freund＇ s adjuvant （CFA） induced conditioned place avoidance （C-CPA）, which has been set up in our previous studies. Methods： The electrode was self-made and the CPA responses were recorded by in vivo multi-channel recording method. Results： ①The electrical activities of ACC neurons could be successfully recorded by the self-made electrode. ②Before or after the injection of CFA, rats were respectively conditioned to the different place. The firing rates of ACC neurons in the CFA-paired place vs that in the non- CFA-paired place was （0.853 ± 1.377） imp/s vs （0.221 ± 0.971） imp/s （ P 〈 0.05, n = 26）. ③The CPA responses in the CFA-paired place vs that in the non-CFA-paired place were （303.55 ± 61.77）s vs （140.32± 33.52）s（ P 〈 0.05, n = 6）. Conclusion： The firing rates of rACC（rostral Anterior Cingulate Cortex） neurons were involved in the occurrence of the affective pain.

关 键 词：在体多通道电生理学记录 完全弗氏佐剂 条件位置回避 痛情绪 前扣带皮层 

分 类 号：R338[医药卫生—人体生理学]