石墨烯量子点对大鼠造血系统的影响  

The effects of graphene quantum dots on hematopoietic system in rats

作　　者：王婷健 王莎莉[1] 

机构地区：[1]重庆医科大学生理教研室,重庆400016

出　　处：《中国应用生理学杂志》2016年第1期60-64,I0014,共6页Chinese Journal of Applied Physiology

摘　　要：目的:研究石墨烯量子点(GQDs)对大鼠造血系统的影响。方法:30只SD雄性大鼠随机分为3组(n=10):对照组、高、低剂量GQDs实验组(10 mg/kg·d,5 mg/kg·d),对照组尾静脉注射等容积生理盐水,实验组尾静脉注射GQDs,连续28 d。麻醉处死,心脏取血,检测血常规及肝肾功能,获取骨髓单个核细胞(BMCs),流式细胞仪检测细胞周期及凋亡情况。另取3只健康雄性SD大鼠体外培养大鼠四肢骨髓单个核细胞,GQDs分别作用24 h,48 h,72 h后,CCK-8检测细胞增殖情况,ELISA检测细胞上清液中粒-巨细胞刺激集落因子(GM-CSF)含量。结果:GQDs在10 mg/kg·d剂量范围内,实验组大鼠红细胞数和血红蛋白浓度显著增高(P<0.05);白细胞和淋巴细胞有增高的趋势;骨髓单个核细胞周期DNA合成前期(G1期)缩短(P<0.01),DNA合成期(S期)显著延长(P<0.01),细胞凋亡无明显影响;甘油三酯和高密度蛋白浓度显著降低(P<0.01)。GQDs作用72 h大鼠骨髓单个核细胞明显增殖(P<0.05),细胞上清液中GM-CSF含量显著增加(P<0.01)。结论:一定浓度的GQDs可促进大鼠造血功能。Objective： To study the effects of graphene quantum dots（GQDs）on hematopoietic system in rats. Methods： Thirty male SD rats were randomly divided into three groups （n = 10） ：control group, high dose group（ 10 mg/kg· d）, low dose group（5 mg/kg· d）, The rats in experimental group were intravenous injected with GQDs for 28 days and those in control group were injected with normal saline at the same volume. Routine blood and the function of liver and kidney were detected by instrument analysis. The cycle and apoptosis of bone marrow mononuclear cells（BMCs） were detected by FCM. The other three only healthy male SD rat bone marrow manonuclear cells（BMCs） were cultured by joining GQDs for 24 h,48 h,72 h in vitro, the proliferation was assayed by CCK-8, the content of granulocyte macrophage colony stimulating factor （GM-CSF） from cultural supernatants were detected by ELISA. Results： The amount of red blood cell and concentration of hemoglobin from experimental group were increased significantly compared with those of control groups （ P 〈 0.05）, the concentration of triglyceride and high density lipoprotein were decreased. DNA synthesis period was prolonged（ P 〈 0.01）, there was no significant difference in apoptosis. BMCs were promoted proliferation clearly after using GQDs for 72 h（ P 〈 0.05）. The content of GM-CSF was increased（ P 〈 0.01）. Conclusion： GQDs may promote hematopoietic function in rats.

关 键 词：石墨烯量子点 造血系统 骨髓单个核细胞 大鼠 

分 类 号：Q599[生物学—生物化学]