高效液相色谱法同时测定开心散提取物中3种主要成分的含量  

Determination of Three Major Components in Kaixin San by HPLC

作　　者：张静[1,2] 穆丽华[2] 赵润清[2,3] 李牧函[2,4] 王东晓 刘屏[2] 

机构地区：[1]山西中医学院中药学院,太原030024 [2]中国人民解放军总医院,北京100853 [3]河北北方学院,河北张家口075000 [4]北京中医药大学中药学院,北京100029

出　　处：《中国实验方剂学杂志》2016年第4期65-68,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81274180)

摘　　要：目的:建立HPLC-ELSD同时测定开心散提取物中主要抗抑郁类成分tenuifoliside A,1-O-(E)-benzoyl-[3-O-(E)-alphatolluyl]-β-D-fructofuranosy-(2→1)-[β-D-glucop-yranosyl-(1→2)]-α-D-glucopyranoside和人参皂苷Rb_1含量的方法。方法:采用HPLC-ELSD分析方法,Agilent HC C_(18)色谱柱(4.6 mm×250 mm,5μm),柱温25℃,流动相乙腈-0.65%乙酸铵水溶液进行梯度洗脱,流速1.0 m L·min^(- 1),漂移管温度115℃,载气流量3.2 L·min^(- 1),气体空气。结果:tenuifoliside A,1-O-(E)-benzoyl-[3-O-(E)-alphatolluyl]-β-D-fructofuranosy-(2→1)-[β-D-glucop-yranosyl-(1→2)]-α-D-glucopyranoside和人参皂苷Rb_1分别在1.113~10.980,1.581~15.726,0.712~7.128μg呈现良好的线性关系;精密度、稳定性和重复性均符合定量分析要求;三者的平均回收率分别为98.36%,99.58%和98.97%;百分含量分别为0.999%,1.670%和0.579%。结论:该分析方法简便、可靠,可用于开心散提取物中tenuifoliside A,1-O-(E)-benzoyl-[3-O-(E)-alphatolluyl]-β-D-fructofuranosy-(2→1)-[β-D-glucopyranosyl-(1→2)]-α-D-glucopyranoside和人参皂苷Rb_1的含量测定,且可以作为开心散质量控制方法之一。Objective： To found a quantitative HPLC-ELSD method for determining tenuifoliside A, 1- O- （E） -benzoyl- [3-0- （E） -alphatolluyl] β-D-fructofuranosy- （2 - 1） - [β-D-glucop-yranosyl- （1 2） ] -a-D-glucopyranoside and ginsenoside Rb1 from Kaixin San, which are used for anti-tumor. Method： The procedure of HPLC-ELSD was performed on the chromatographi column of Agilent HC C18 （4.6 mm x 250 mm, 5 μm）at 25 ℃ , and the mobile phase was acetonitrile-0.65% ammonium acetate solution in gradient elution. The flow velocity was 1.0 mL·min-1. The temperature of driti tube was 115℃ and the gas was air, flow rate was 3.2mL·min-1. Result： Tenuifoliside A showed a good linear relationship at a range from 1. 113 μg to 10. 980 μg, 1-0- （E） -benzoyl- [ 3-O- （E） -alphatolluyl ] -β-D-fructofuranosy- （ 2 - 1 ） [β-D-glucop-yranosyl- （ 1 2） ] -a-D-glucopyranoside, from 1. 581 p,g to 15. 726 μg, and ginsenoside Rba, from 0. 712μg to 7. 128μg; Precision, stability and repeatability are comply with the requirement of quantitative analysis. The average recovery was respectively 98.36% , 99.58% and 98.97%. The percentage composition was respectively 0. 999% , 1. 670% and 0. 579%. Conclusion： The method was simple and reliable, which can be used for the assaying of tenuifoliside A, 1-0- （E） -benzoyl-β-0- （E） -alphatolluyl ] -β-D-fructofuranosy- （ 2-→ 1 ） β-D-glucop- yranosyl- （1-→2）] -ol-D-glucopyranoside and ginsenoside RbI as well as quantity control of Kaixin San.

关 键 词：开心散 寡糖酯类 人参皂苷RB1 高效液相色谱-蒸发光散射 抗抑郁 

分 类 号：R284.1[医药卫生—中药学]