机构地区:[1]广东省疾病预防控制中心卫生毒理所,广州511430 [2]中山大学公共卫生学院,广州510080 [3]暨南大学医学院,广州510632
出 处:《中国实验方剂学杂志》2016年第4期89-94,共6页Chinese Journal of Experimental Traditional Medical Formulae
基 金:国家高技术研究发展计划(863计划)项目(2010AA023001);广东省科技计划项目(2013B010404033);广东省自然科学基金项目(S2013010013289);广州市科技计划项目(201300000161);广东省中药局课题(20132108;20121275)
摘 要:目的:研究夏枯草对小鼠急性束缚应激损伤的保护作用和主要功效成分分析。方法:将雄性Balb/C小鼠分为正常组,束缚模型组,1.25,2.50,7.50 g·kg^(-1)夏枯草干预组。连续给药5 d后,束缚模型组和夏枯草干预组小鼠急性束缚2 h,所有小鼠分别检测脑组织的总活性氧(总ROS),过氧化氢(H_2O_2),丙二醛(MDA),8羟基鸟嘌呤(8-OHd G)和蛋白质羰基含量,并观察超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性。以硝酸铝显色法测定总黄酮,香草醛显色法检测总三萜,硫酸-苯酚法测量总多糖含量,并以ORAC法分别检测3种物质的总抗氧化能力。结果:与正常组比较,2 h急性束缚可增加小鼠脑组织中的H_2O_2,MDA,8-OHd G,蛋白质羰基含量,降低SOD酶活性(P<0.05,P<0.01),但总ROS含量和GSH-Px酶活性变化不明显。与束缚模型组比较,夏枯草能减少小鼠脑组织中H_2O_2,MDA和蛋白质羰基含量,增强SOD酶活性(P<0.05,P<0.01)。夏枯草的总黄酮、总三萜和总多糖含量分别为8.91,2.45,10.16 mg·g^(-1)。总黄酮、总三萜和总多糖ORAC结果分别为(127.5±1.0),(45.75±2.5),(2.25±0.75)mmol·L^(-1)。结论:夏枯草对急性束缚应激诱发小鼠氧化损伤具有一定的保护作用,黄酮可能是抗氧化应激作用的主要功效成分。Objective: To study major functional components of Prunellae Spica and its protective effect against the oxidative injury induced by acute restraint stress in mice. Method: The male Balb/C mice were randomly divided into negative group and model control group, and 1.25, 2.50, 7.50 g·kg-1 Prunellae Spica intervention groups. Mice in the intervention groups were given Prunellae Spica by gavage for 5 days, mice in model control group and negative group were given distilled water. After the last administration, intervention groups and model control group received 2 h acute restraint stress, and then the mice in the groups were sacrificed. The following anti-oxidative indicators in brain tissues were detected respectively: oxidative stress reactive oxygen species (ROS), hydrogen peroxide (H202), malondialdehyde (MDA), 8-hydroxy-deoxyguanosine (8-OHdG),protein carbonyl content, superoxide dismutase (SOD) activity and glutathione peroxidase (GSH-Px) activity. We analyzed main components of prunella, including total flavonoids (aluminum nitrate chromogenic method) , total triterpenoids (vanillin colorimetric method) and total polysaccharides (phenol-sulfuric acid method), and detected their total antioxidant capacity (ORAC). Result : Compared with the negative group, content of H202 , MDA, 8- OHdG and protein carbonyl in model group significantly increased, while SOD activity obviously decreased (P 〈 0.05, P 〈 0.01 ). No obvious difference was found in ROS and GSH-Px contents. Compared with the model control group, SOD activity in the three intervention groups was increased (P 〈 0.05, P 〈 0.01 ), content of H2O2, MDA and protein carbonyl were much decreased. Total flavonoids content was 8.91 mg·g-1, total triterpene content was 2.45 mg·g-1, and total polysaccharide content was 10. 16 mg·g-1 Total ORAC values of total flavonoids, total triterpenoids and polysaccharides were ( 127.5± 1.0), (45.75 ±2.5 ), (2.25± 0.75 ) mmol·L-1, respectively
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