从免疫角度探讨黄芪桂枝五物汤对小鼠衰老皮肤瘙痒模型的影响  

Effects of Huangqi Guizhi Wuwu Tang in Senile Pruritus Mouse Model from Perspective of Immunology

作　　者：赵乐[1] 李艳彦[1] 

机构地区：[1]山西中医学院基础医学院,太原030024

出　　处：《中国实验方剂学杂志》2016年第4期127-131,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：山西省卫生厅科技攻关项目(201201101)

摘　　要：目的:研究黄芪桂枝五物汤对小鼠衰老皮肤瘙痒的作用,并从免疫学角度探讨衰老皮肤瘙痒的发病机制。方法:昆明种小鼠84只,随机分为7组,每组12只,分别为正常组,皮肤衰老组,皮肤衰老瘙痒模型组,苯海拉明组(0.65 mg·kg^(-1)),黄芪桂枝五物汤低、中、高剂量组(3.25,6.5,13 g·kg^(-1)),除正常组外,其余各组采用D-半乳糖125 mg·kg^(-1)连续颈背部皮下注射42 d建立皮肤衰老小鼠模型,在此基础上,行尾静脉注射右旋糖酐注射液1.25 mg·kg^(-1),复制出衰老皮肤瘙痒模型。观察黄芪桂枝五物汤ig给药7 d后对小鼠皮肤瘙痒反应的瘙痒潜伏时间及瘙痒次数。酶联免疫吸附法测定血清白细胞介素1β(IL^(-1)β),IL-4,蛋白酶激活受体-2(PAR-2),P物质(SP),显微镜下计数肥大细胞数目。结果:与正常组比较,皮肤瘙痒组及皮肤衰老瘙痒模型组瘙痒潜伏时间降低,及瘙痒次数明显升高,小鼠血清IL^(-1)β,PAR-2,P物质表达明显升高,PAR-2含量明显降低(P<0.05);黄芪桂枝五物汤能明显延长衰老皮肤瘙痒小鼠瘙痒潜伏搔抓时间(P<0.05),减轻皮肤瘙痒小鼠搔抓次数(P<0.05)。用药后,各用药组小鼠血清IL^(-1)β,PAR-2,P物质表达较衰老皮肤瘙痒模型组降低(P<0.05),IL-4水平较衰老皮肤瘙痒模型组显著升高(P<0.05),肥大细胞较衰老皮肤瘙痒模型组显著性减少(P<0.05)。结论:黄芪桂枝五物汤对衰老皮肤瘙痒模型作用显著,其减轻皮肤瘙痒的作用与调节免疫失衡状态有关。Objective： To study the treating effect of Huangqi Guizhi Wuwu Tang in senile mouse with pruritus disease, and its possible mechanism from the perspective of immune imbalance. Method： Totally 84 Kunming mice were randomly into the normal group, the skin aging group, the skin aging pruritus model group, the diphenhydramine group （0.65 mg·kg-1）, and low, middle and high-dose Huangqi Guizhi Wuwu Tang group （3.25, 6.5, 13 g·kg-1） , with 12 mice in each group. Except for the normal group, all of the remaining groups were injected with D-galactose 125 mg·kg-1 through mouse neck and back for consecutively 42 days to build the senile mouse model; based on that, the senile pruritus model was induced by injecting dextran 1.25 mg·kg-1 through mouse caudal vein. Seven days later, mouse＇s scratching frequency and the scratching latency time of were observed with digital video camera. Then the content of interleukin-1/3 （IL-1/3）, IL-4, protease-activated receptor-2 （PAR-2） and substance P in serum were determined the enzyme linked immunosorbent assay, and the mast cells number in skin was counted under microscope. Result： Compared with the normal group, the skin pruritus group and skin aging pruritus model group showed signficiant decreases in scratching latency time and PAR-2 conent and increases in scratching frequency and serum IL-lfl, PAR-2 and substance P expression （P 〈 0.05）. Huangqi Guizhi Wuwu Tang can significantly extend the latent scratching time （P 〈 0.05 ） and decrease the scratching frequency （P 〈 0.05）. After intragastric administration, contents of IL-1, PAR-2 and substance P in serum of drug-treated mouse groups were lower than that of the skin aging pruritus model group （P 〈 0.05 ） , while IL-4 level in serum was significantly higher than the senile pruritus mouse model group （P 〈 0.05）. Meanwhile, mast cells number is decreased （P 〈 0.05） compared with the senile pruritus mouse model group. Conclusion： Huangqi Guizhi Wuwu Tang has prominent t

关 键 词：黄芪桂枝五物汤 老年性皮肤瘙痒 瘙痒次数 免疫失衡 

分 类 号：R285.5[医药卫生—中药学]