Foxp3和RORγt在肝泡型包虫病患者肝脏组织中的表达及意义  

Expression and effect of Foxp3 and RORγt in liver tissue from alveolar echinococcosis patients

作　　者：张恒[1,2] 吐尔洪江.吐逊 马海长[1,2] 沙地克.阿帕尔 林仁勇[1] 温浩[1,2] 

机构地区：[1]新疆医科大学第一附属医院医学研究中心新疆包虫病基础医学重点实验室,新疆乌鲁木齐830054 [2]新疆医科大学第一附属医院消化血管外科中心肝(移植)腹腔镜外科,新疆乌鲁木齐830054

出　　处：《中国现代普通外科进展》2016年第1期1-5,共5页Chinese Journal of Current Advances in General Surgery

基　　金：国家自然科学基金项目(81160201)

摘　　要：目的:探讨Foxp3和RORγt在肝泡型包虫病患者肝脏组织中的表达及意义。方法:将手术治疗的14位肝泡型包虫病患者的肝脏组织分为3组:病灶组织组(L组)14例,病灶旁组织组(P组)14例,正常肝组织组(N组)14例。应用实时荧光定量PCR(q RT-PCR)法检测各肝脏组织中Foxp3和RORγt m RNA的表达,分析其结果。结果:Foxp3 m RNA在L组中的表达明显高于P组(P=0.005)和N组(P=0.004),P组中的表达高于N组(P=0.246)。RORγt m RNA在L组中的表达高于P组和N组(L组vs P组,P=0.187);L组vs N组,P=0.044),在P组中的表达高于N组(P=0.027)。肝脏不同组织中,RORγt与Foxp3的表达量呈正相关。结论:Th17细胞转录因子Foxp3和Treg细胞转录因子RORγt在肝脏病灶组织中m RNA的表达升高,这些变化可能与泡型包虫病发病过程中寄生虫肉芽肿的形成有关。Objective： To investigate the expression and effect of Foxp3 and RORrt in liver tissue from Alveolar Echinococcosis patients. Methods： All samples were selected from 14 patients ith hepatic AE who were treated at the First Affiliated Hospital of Xinjiang Medical University.Diveded the tissues into 3 groups： 14 were lesion tissues（L）, 14 were para-lesion tissues（P）and14 were normal liver tissues（N）. The tissues expression levels of Foxp3 and RORrt gene were measured by real-time PCR（q RT-PCR）. For statistical analysis, Student＇s t test and Pearson correlation were used. Results： The hepatic Foxp3 levels in the group L were significantly higher than that in group P（t=3.778, P=0.005）and group N（t=3.990, P=0.004）. There was no significant difference between group P and group N（t=1.252, P=0.246）, Meanwhile, RORγt gene expression levels in group P were also significantly higher than that in group N（t=2.705, P=0.027）,but there was no significant difference between group L and group P（t=1.355, P=0.187）. And there was also have significant difference between group L and group N（t=2.261, P=0.044）. And the expression levels of RORγt and Foxp3 is positive correlate in different liver tissue. Conclusion： The expression of Th17 transcription factor RORrt and Treg transcription factor Fox P3 are increased in hepatic lesion, they may involve the process of parasitic granuloma formation.

关 键 词：棘球蚴病 肝 寄生虫感染 FOXP3 RORΓT 

分 类 号：R532.32[医药卫生—内科学] R657.3[医药卫生—临床医学]