一株生防细菌GB58的鉴定与抑菌能力测定  

Identification and Antimicrobial Broad-spectrum Analysis of a Biocontrol Bacteria Strain GB58

作　　者：卢钰升[1] 顾文杰[1] 蒋瑞萍[1] 孙丽丽[1] 徐培智[1] 谭志远[2] 解开治[1] 李文英[1] 李夏[1] 

机构地区：[1]广东省农业科学院农业资源与环境研究所农业部南方植物营养与肥料重点实验室广东省养分资源循环利用与耕地保育重点实验室,广州510642 [2]华南农业大学农学院,广州510642

出　　处：《中国农学通报》2016年第6期198-204,共7页Chinese Agricultural Science Bulletin

基　　金：广东省科技计划项目"基于菜园退化土壤的功能性微生物接种剂研发与应用"(2013B020310001);广东省科技计划项目"橄榄有机无机专用肥的研制与应用"(2013B020419009);广东省科技计划项目"广东省新型肥料与高效施肥工程技术研究中心建设"(2014B090904068);广东省科技计划项目"广东省新型肥料科技创新与服务平台建设";广东省财政厅项目"多功能生物复合型有机营养液肥的研发与应用";广州市农业局农业科技攻关招标项目"城郊结合处集约化畜禽养殖场固体废弃物无害化与资源化综合利用研究"(GZCQC0902FG06017)

摘　　要：对从广东水稻土中获得能高效抑制植物病原菌的细菌GB58进行鉴定、抑菌广谱性测定,明确其潜在应用价值。采用平板对峙法测定GB58抑菌广谱性,并参照细菌系统鉴定方法,通过形态、生理生化、分子生物学手段鉴定菌株。抗菌广谱性试验结果表明:GB58对多达12种的植物真菌性病害和1种细菌性病害具有较好的拮抗作用。细菌常规鉴定试验结果显示:GB58为芽孢杆菌属,碳源利用分析表明GB58能够利用46种碳源物质以及耐酸耐盐,通过16S r RNA、gyr A序列进行系统发育分析,确定GB58为解淀粉芽孢杆菌。GB58是一株抗菌范围广,营养竞争能力强的生防细菌,具备适应华南酸性土壤复杂环境的繁殖和代谢能力。This article presented the identification and antimicrobial broad-spectrum analysis of the biocontrolbacteria strain GB58 obtained in rice soil of Guangdong Province, which could inhibit plant pathogens, andaimed to clarify its potential application value. The antibacterial broad-spectrum of GB58 was determined bythe plate confrontation method, and the bacterial strain was identified by morphological, physiological,biochemical and molecular biology methods which were the reference for bacteria system identification. Theantimicrobial broad-spectrum result showed that GB58 had better antagonism to twelve plant fungal diseasesand one plant bacterial disease. In the meantime, common bacterial identification test showed that GB58 wasbacillus and the carbon utilization analysis showed that GB58 could utilize 46 kinds of carbon sources and wasable to tolerate acid and salt. Moreover, the phylogenetic analysis through 16 S r RNA and gyr A sequenceconcluded that GB58 was bacillus amyloliquefaciens. All the results suggested that GB58 was a biocontrolbacteria strain with wide antibacterial range and high nutrition competitive ability, which had the ability ofreproduction and metabolism that enabled it to adapt to southern China’s acid-soil-dominant environment.

关 键 词：生防细菌 鉴定 系统发育 抗菌广谱性 

分 类 号：S182[农业科学—农业基础科学] S432.4