浅谈林木体细胞融合技术  

Discussion on Somatic Fusion Technology in Forest

作　　者：孙宇涵[1] 王欢[1] 李云[1] 

机构地区：[1]北京林业大学林木育种国家工程实验室/林木花卉遗传育种教育部重点实验室/北京林业大学生物科学与技术学院,北京100083

出　　处：《中国农学通报》2016年第4期136-143,共8页Chinese Agricultural Science Bulletin

基　　金：国家林业局重点项目"杉木诱变育种技术"(2012-06);国家"863"项目"杉木;马尾松分子育种及品种创制"(2011AA100203);国家自然科学基金项目"刺槐杂交结实率低的机理研究"(31170629)

摘　　要：为了研究利用体细胞融合技术培育新型林木品种,综述了国内外近10多年的林木原生质体培养、体细胞融合技术以及其在林木育种工作中的应用研究,发现前人在原生质体的材料选择、解离和培养技术方法上获得了较大的进展,并在体细胞融合方法、方式和后期杂种细胞筛选方面取得了丰硕的成果,但相关研究还存在不足,今后还需在林木体细胞融合技术的可融合物种范围、影响因素和生理生化机制等方面开展更加深入的系统研究。The paper aims to study how to cultivate the new forest varieties by the somatic fusion technology.For this purpose, the recent 10 years＇ research on the protoplast culture, somatic fusion technology and itsapplication in forest breeding were summarized. Through the analysis of previous studies, it was found that theresearch on protoplast culture of forest had gained great progress in the material selection, dissociation andculture, and the research on somatic fusion technology had also achieved positive results on the fusion methodand the selection of somatic hybrids. But the related research was still insufficient. In the future, it is necessaryto carry out the in-depth study on forest somatic fusion technology in the perspective of fusion species,influencing factors and physiological and biochemical mechanism.

关 键 词：原生质体培养 体细胞融合 林木育种 变异 

分 类 号：S722.37[农业科学—林木遗传育种]