β-甘露聚糖酶高产菌株的紫外线诱变选育  

Screening of β-mannanase High Yield Strain by Ultraviolet Mutation

作　　者：周晓杭[1,2] 杜春梅[2] 葛菁萍[2] 

机构地区：[1]牡丹江医学院基础医学院病原与免疫学实验室,黑龙江牡丹江157000 [2]黑龙江大学生命科学学院微生物黑龙江省高校重点实验室,哈尔滨150080

出　　处：《中国农学通报》2016年第5期23-27,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"菌群演替与温水沤麻系统关键酶产生菌代谢组学特征的耦合机制"(31270534);黑龙江省自然科学基金"侧孢芽孢杆菌与枯草芽孢杆菌共培养代谢产物的研究"(C201203);黑龙江大学高层次人才(创新团队)支持计划"功能微生物选育及其产物应用"(Hdtd2010-17);黑龙江省科技厅"农业微生物发酵技术"科技创新团队

摘　　要：为给生产实践提供具有更高产酶量的优良菌株,本研究以实验室保藏的一株地衣芽孢杆菌HDYM-04为原始出发株,经自然筛选、紫外线诱变,选育出一株β-甘露聚糖酶高产菌株U2-12。结果表明:依据致死率所选用的最佳诱变剂量为照射距离50 cm,照射时间为10 s,此时,菌株的总突变率为76.11%,正突变率为54.60%,负突变率为20.92%。地衣芽孢杆菌U2-12的产β-甘露聚糖酶活性是原始未经紫外线诱变的出发株的242.19%,其遗传性状稳定。本研究可以为后续试验及生产实践提供优势产酶菌株,并为研究紫外线对β-甘露聚糖酶产酶基因的影响提供先决条件。In order to provide strains which have a high enzyme yield, the original strain Bacillus licheniformis HDYM-04 was isolated naturally and then treated with ultraviolet ray. The best mutation condition wasdetermined to expose the strain to the ultraviolet source at 50 cm for 10 s based on the fatality rate. The totalstrain mutation rate was 76.11%, the positive mutation rate was 54.60%, and the negative mutation rate was20.92%. A stable β-mannanase producing strain Bacillus licheniformis U2-12 was obtained with the enzymeactivity being 242.19% of the original strain. The results can provide superior enzyme production strains forthe following test and production practice, and references for studying the effect of ultraviolet ray on β-mannanase gene.

关 键 词：地衣芽孢杆菌 Β-甘露聚糖酶 紫外线诱变 

分 类 号：Q939.99[生物学—微生物学]