家蚕雄dsx^(M3)异位表达对下游靶基因表达的影响  

Ectopical Expression of Silkworm Male-specific dsx^(M3) and Its Effect on Downstream Targets

作　　者：段建平[1,2] 李莹[2] 阚云超[2] 姚伦广[2] 夏庆友[1] 

机构地区：[1]西南大学家蚕基因组生物学国家重点实验室,重庆400716 [2]南阳师范学院河南省伏牛山昆虫生物学重点实验室,南阳473061

出　　处：《中国生物工程杂志》2016年第2期38-42,共5页China Biotechnology

基　　金：国家"973"计划(2012CB114600);国家自然科学基金(31501900);高层次人才专项项目(70639)资助项目

摘　　要：家蚕双性基因dsx通过雌雄特异选择性剪接并表达影响体细胞性别发育。在对Bmdsx的表达情况进行深入分析时发现了一个新的雄特异剪接体dsx^(M3),但其功能未知。为揭示Bmdsx^(M3)的生物学功能,首次通过转基因在雌蚕中异位表达Bmdsx^(M3),成功获得两个转基因品系T1和T2。在T1和T2中虽未出现发育异常和性反转事件,但在转基因雌性个体中,SP1和Vg的表达量都出现明显下调,而PBP的表达量也出现明显上调,表明新剪接体Bmdsx^(M3)能够调控Bmdsx下游靶基因的表达,暗示Bmdsx^(M3)同样参与家蚕体细胞性别发育。Doublesex gene dsx controls the somatic sexual development by sex-specifically alternative splicing. The expression of Bmdsx was analysed and a novel male-specific splice form BmdsxM3 was also found, but whether it has biological function is unknown. Here, two transgenic lines T1 and T2 were constructed to ectopically express BmdsxM3 in females. As a result, sex reversal and development abnormality all failed to tum up,but the expression levels of SP1 and Vg were downregulated in the fat body of transgenic females, and the expression level of PBP was upregulated in the antenna of transgenic females. All the results indicated that the novel splice form BmdsxM3 had the role of regulation of downstream target gene expression, and it took part in the somatic sexual development in silkworm,Bombyx mori.

关 键 词：家蚕 双性基因 转基因 靶基因 

分 类 号：Q789[生物学—分子生物学]