RNA原位杂交技术在玉米研究中的应用  

The Application of RNA In Situ Hybridization Technique in Maize

作　　者：李洁[1] 于明[1] 邴杰[1] 周晓今[2] 

机构地区：[1]北京师范大学生命科学学院,北京100875 [2]中国农业科学院生物技术研究所,北京100081

出　　处：《植物生理学报》2015年第12期2280-2286,共7页Plant Physiology Journal

基　　金：国家自然科学基金青年科学基金项目(31101095);北京师范大学教师发展基金项目

摘　　要：RNA原位杂交技术是在组织显微水平研究目标基因时空表达分布的重要技术手段。在植物中,原位杂交技术广泛应用于拟南芥和水稻的研究,而在玉米的研究中该技术应用较晚,实验技术体系尚有待优化和完善。文章以玉米为材料探索和优化原位杂交实验技术体系,并着重对实验的方法步骤、技术要点、结果分析等方面进行讨论和总结。实验结果表明,优化后的实验技术体系能够特异的检测到目标基因在玉米雄花中表达分布。RNA in situ hybridization technique is important in the detection of the temporal and spatial expression of functional genes at a histological level. In situ hybridization technology has been widely used in studies towards Arabidopsis and rice, but few studies have been reported in maize, possibly due to its technique difficulties. In this article, the authors explored and optimized the experimental system of in situ hybridization technique by using different maize tissues, especially summarized the method and steps, the key points of technology,typical results, etc. The results suggest that the optimized in situ hybridization technique is able to specifically detect the target gene in the tassel of maize.

关 键 词：RNA原位杂交 基因表达 玉米 

分 类 号：S513[农业科学—作物学]