山东沂源羊体寄生长角血蜱携带病原调查研究  

Research on the pathogenic infection of Haemaphysalis longicornis ticks parasitizing goats in Yiyuan County, Shandong Province

作　　者：刘相叶[1] 郑辰[1] 李向阳[1] 颜超[1] 华慧[1] 汤仁仙[1] 郑葵阳[1] 

机构地区：[1]徐州医学院病原生物学与免疫学教研室、感染与免疫实验室,江苏徐州221004

出　　处：《徐州医学院学报》2015年第12期837-840,共4页Acta Academiae Medicinae Xuzhou

基　　金：中国博士后科学基金（2015M580472）;江苏省自然科学基金青年基金（BK20150212）;徐州医学院优秀人才科研启动基金（D2015006）;江苏省脑病生物信息重点实验室开放课题（JSB11402）

摘　　要：目的：了解山东省沂源县境内寄生于山羊体表的长角血蜱携带病原体的情况。方法2014年9月—2015年7月从该地区山羊体表采集不同生活史时期的长角血蜱。利用PCR扩增特异性基因的方法，分别检测长角血蜱感染嗜吞噬细胞无形体、巴贝斯虫、伯氏疏螺旋体以及巴尔通体的情况。结果山东沂源地区长角血蜱中嗜吞噬细胞无形体的感染率为48．1％，巴贝斯虫的感染率为40．7％，其中24．1％的长角血蜱存在嗜吞噬细胞无形体和巴贝斯虫的双重感染，但所有长角血蜱体内未检测到伯氏疏螺旋体和巴尔通体。结论山东沂源羊体寄生的长角血蜱存在较高的嗜吞噬细胞无形体和巴贝斯虫感染率。因此，该地区应加强对人粒细胞无形体病和巴贝斯虫病的防控。Objective To investigate the tick-borne pathogens transmitted by Haemaphysalis longicornis ticks par-asitizing goats in Yiyuan County, Shandong province.Methods H.longicornis ticks were collected from goats from Sep-tember 2014 to July 2015.Through amplification of specific genes by PCR, tick-borne pathogens including Anaplasma phagocytophilum, Babesia species, Borellia burgdorferi and Bartonella species were detected.Results The infection rates of A.phagocytophilum and Babesia sp.in H.longicornis ticks were 48.1%and 40.7%, respectively.Meanwhile, 24.1% of H.longicornis ticks were infected with both A.phagocytophilum and Babesia sp.However, neither Borellia burgdorferi nor Bartonella sp.was detected in the ticks.Conclusion High infection rates of A.phagocytophilum and Babesia sp.were found in H.longicornis ticks parasitizing in goats in Yiyuan County.Therefore, urgent measures should be promptly taken to enhance the prevention and control of human granulocytic anaplasmosis and babesiosis.

关 键 词：长角血蜱 嗜吞噬细胞无形体 巴贝斯虫 伯氏疏螺旋体 巴尔通体 

分 类 号：R384.4[医药卫生—医学寄生虫学]