口服维生素D对实验性脑疟小鼠树突状细胞的影响  

Effects of Pre-Treatment with Vitamin D on Dendritic Cells In Experimental Cerebral Malaria Mouse

作　　者：杨志蕃[1] 庞维[2] 郁春云 吕衍民 曹雅明[2] 

机构地区：[1]沈阳军区总医院肾内科,辽宁沈阳110016 [2]中国医科大学基础医学院免疫学教研室,辽宁沈阳110122

出　　处：《微生物学杂志》2015年第6期74-77,共4页Journal of Microbiology

基　　金：高等学校博士学科点专项科研基金项目(20112104110018)

摘　　要：维生素D(VD)为固醇类衍生物,除发挥抗佝偻病作用,还具有一定的免疫调节功能。主要研究VD对实验性脑疟小鼠树突状细胞的影响。结果显示,与对照组相比,在伯氏疟原虫(P.b ANKA)感染前给予VD,可降低C57BL/6小鼠发生脑型疟疾的风险,存活时间明显延长。VD预处理后,脾脏中髓样树突状细胞(CD11c+CD11b+)和浆样树突状细胞(CD11c+B220+)百分率明显降低,CD11c+MHCII+细胞、CD11c+TLR4+细胞和CD11c+TLR9+细胞的百分含量也显著减少。由此提示,预防性口服VD可抑制感染小鼠树突状细胞的数量和功能,对脑型疟疾的发生具有一定预防作用,为新型抗疟药物的研发提供参考。Vitamin D （VD） is the steroid derivatives, it has the effect of anti-rickets, besides a certain immune function. The main immunomodulatory effects and mechanisms of VD on experimental cerebral malaria mouse were studled. The results showed that, as compared with control group, the C57BL/6 mice that administered VD prior to P. berghel ANKA （ P. b ANKA） infection, could prevent from cerebral malaria and the survival time was significantly prolonged. The percentages of myeloid dendritic cells （CDllc^＋ CD11b^＋ cells） and plasmacytoid dendritic cells （CDllc^ ＋ B220^ ＋ cells） in spleen were significantly redueed after pretreatment of VD. While the percentages of CD11c^ ＋ MHCII^＋ cells, CD11c^ ＋ TLR4^ ＋ cells and CD11c^＋ TLR9^＋ cells in spleen were also decreased obviously. Thereby suggested that preventively oral VD could inhibit the number and function of dendritic cells, and had a preventive effect for cerebral malaria mice in C57BL / 6 infected P. b ANKA. This provided a theoretical basis for the research and development of new anti-malarial medicines.

关 键 词：脑型疟疾 维生素D(VD) 免疫调节 树突状细胞 

分 类 号：Q939.93[生物学—微生物学] R254.5[医药卫生—中医内科学]