激素抵抗型哮喘小鼠动物模型的实验性研究  

Experimental Study of Steroid Resistant Asthma Animal Models in Mice

作　　者：文莎[1] 吴峰[1] 

机构地区：[1]福建医科大学附属协和医院呼吸科,福州350001

出　　处：《福建医科大学学报》2015年第4期211-214,共4页Journal of Fujian Medical University

摘　　要：目的探索建立激素抵抗型支气管哮喘小鼠动物模型的有效方法。方法普通级BALB/C雌性小鼠30只,随机均分为5组。正常对照(NC)组直接处死,非治疗哮喘(NTA)、激素治疗敏感哮喘(SSA)、气管重塑(AR)、激素抵抗型哮喘(SRA)组均采用卵清白蛋白(OVA)致敏、激发造模,SSA、SRA组予地塞米松(DEX)干预,AR、SRA组连续雾化9周。行肺泡灌洗液(BALF)细胞计数、肺组织切片细胞分类计数、气管壁及平滑肌厚度图像分析。结果 SRA组在实验末期出现持续性喘息,DEX干预后喘息不缓解。NTA、AR、SRA组肺内炎症细胞浸润程度、气管壁程度较NC组严重,SRA组肺内嗜酸性粒细胞、淋巴细胞浸润及AR程度较SSA组严重(P<0.05),AR组BALF细胞计数及AR程度较NTA组严重(P<0.05),SRA组与AR组比较,仅肺内嗜酸性粒细胞数明显升高(P<0.01)。结论本研究建立SRA小鼠动物模型的方法切实可行。Objective To explore the method to establish effective steroid resistant asthma animal models in mice. Methods group directly put to death. Common female BALB/C mice were randomly divided into groups. NC Mice of NTA, SSA, AR and SRA were sensitized and challenged with OVA. SSA and SRA mice were intraperitoneal injection of dexamethasone. AR and SRA groups chal lengcd nine consecutive weeks. All the mice were sacrificed for determining the concentration of inflam matory cells in the bronchoalveolar lavage fluid （BALF） I.ung tissue samples were taken for counting and classifying infiltrating cells, for anglicizing the thickness of airway wall and smooth muscle At the end of the experiment, persistent wheezing SRA mice appeared, and DEX can＇t cure Results whoop.NTA, AR and SRA groups were more series than NC group in inflammatory cells infiltration in lung and the thickness of airway SRA group was more series than SSA in eosinophils, lymphocyte infiltration in lung and airway remodeling. And AR was more series than NTA in the concentration of inflammatory cells in the BAI,F and airway remodeling （P〈0.05） Compare SRA with AR eosinophils counts increased obviously （P〈0.01）. Conclusion The method in the research for building steroid resistant asthma animal model was reallyfeasible.

关 键 词：支气管 哮喘 小鼠 模型 动物 激素类 气管 

分 类 号：R562.25[医药卫生—呼吸系统] R-332[医药卫生—内科学]