囊胚玻璃化冷冻对雄鼠精子质量的影响  

Effects of blastocyst vitrification on male mice sperm quality

作　　者：孙华[1] 高原[2] 王生存[2] 郁敏燕[1] 郭丰[1] 

机构地区：[1]南通大学附属医院生殖医学中心,江苏226001 [2]南通大学实验动物中心

出　　处：《交通医学》2015年第6期555-558,共4页Medical Journal of Communications

基　　金：南通市科技计划项目(HS2013042);江苏省妇幼保健科研项目(F201442)

摘　　要：目的:研究玻璃化冷冻复苏对子代小鼠精子质量的影响,为玻璃化冷冻对子代生殖安全提供参考。方法:将C57BL/6雌性鼠促排卵后合笼,取3.5天囊胚,玻璃化冷冻至少两周后复苏培养移植。待子代雄鼠性成熟后,处死取睾丸附睾组织,计算睾丸指数,从附睾尾获取精子,制备精子悬液,检测小鼠精子密度、活力、前向运动率及畸形率。结果:玻璃化冷冻复苏的子代雄鼠精子的睾丸指数(0.0078±0.0006g/g)、精子密度[(13.57±3.07)×106/m L]、活力(50.67%±2.03%)、前向运动率(28.87%±2.54%)以及精子畸形率(49.67%±1.21%),与正常C57BL/6雄鼠比较差异无统计学意义(P>0.05)。结论:初步认为囊胚玻璃化冷冻复苏移植对小鼠子代精子功能和形态无明显影响。Objective： To study the effects of blastocyst vitrification on male mice sperm quality, and to provide the reproductive safety reference for the offspring from vitrified blastocyst. Methods： Blastocysts were collected from superovulated C57 BL / 6 female mice and vitrified. Vitrified blastocysts were stored in liquid nitrogen for more than 2 weeks before thawed and transferred to surrogate mice. Offspring of male mice were euthanized after sexual maturity. The testis and epididymis were harvested and weighed, testicular index was calculated. Sperm from epididymis cauda were collected to make sperm suspension, sperm density, progressive motility, total motility and normal morphology rates were studied. Results：There was no significant difference in male mice testicular index（0.0078±0.0006g/g）, sperm density [（13.57±3.07）×10^6/m L], progressive motility（50.67%±2.03%）, total motility（28.87%±2.54%） and normal morphology rates（49.67%±1.21%）between vitrified blastocyst offspring and control C57 BL / 6. Conclusion： Our preliminary results showed that there were no significant effects on male mice offspring sperm function and morphology of blastocyst vitrification.

关 键 词：囊胚 玻璃化冷冻 精子功能 精子形态 

分 类 号：R446.1[医药卫生—诊断学]