支原体肺炎患儿外周血Treg/Th17细胞平衡变化及临床意义  

Changes and the Clinical Significance of the Balance of Treg/Th17 Cells in Peripheral Blood of Children with Mycoplasma Pneumoniae Pneumonia

作　　者：熊远青[1] 黄河[1] 姚霖[1] 王筱璐[1] 唐碧莲[1] 汤慧丽[1] 

机构地区：[1]广东省深圳市福田区人民医院,广东深圳518033

出　　处：《中国医学创新》2015年第36期59-61,共3页Medical Innovation of China

基　　金：国家继续教育发展中心课题项目(2013-065-06-12)

摘　　要：目的:检测不同时期支原体肺炎(MPP)患儿外周血Treg细胞、Th17细胞及其相关细胞因子TGF-β、IL-10、IL-17和IL-6水平,观察其变化规律,探讨MPP急性期免疫失衡发病机制。方法:40例MPP患儿及20例非MP感染性支气管肺炎在急性期(发病<2周)及病程4周时空腹抽取外周静脉血5 m L,使用流式细胞仪检测Treg细胞、Th17细胞的百分率,ELISA方法检测TGF-β、IL-10、IL-17和IL-6细胞因子水平,同时与20例健康体检儿进行比较。结果:急性期MPP组Treg细胞百分率低于正常对照组(P<0.05);病程4周时,MPP组Treg细胞百分率明显提高,MPP组TGF-β、IL-10水平低于非MP感染组及正常对照组(P<0.05)。急性期及病程4周时,MPP组患儿Th17细胞百分率及IL-17、IL-6水平均高于非MP感染组和正常对照组患儿(P<0.05)。结论:儿童MPP感染与免疫调节紊乱密切相关,急性期存在明显的免疫失衡,Treg/Th17细胞失衡及其相关效应因子的表达在儿童MPP感染起到重要的作用。Objective： To observe the percentages of Treg cells and Thl7 cells , the levels of IL-10, TGF- β, IL-17 and IL-6 in peripberal blood of children with mycoplasma pneumoniae pneumonia and explore the role of them in pathogenesis of MPP. Method： 40 children with MPP, 20 Children with non- MPP and 20 healthy children were enrollod.Tbe percentages of Treg cells and Th17 cells in peripheral blood were detected by flow cytometer （ FCM ）, and the levels of IL-10, TGF-β, IL-17 and IL-6 in plasma were determined by ELISA, at the same time, compared with 20 cases healthy children. Result： In the acute stag, The percentage of Treg cells in children with MPP were significantly lower than bealthy infants （ P〈0.05 ） . The percentage of Treg ceils in the 4th week were significantly higher than those in the acute stag, the levels.of IL-10 and TGF-β in children with MPP were significantly lower than those in children with non- MPP and healthy infants in the acute stag and the 4th week （ P〈O.05 ） . The percentage of Th17 ceils and the levels of IL-17 and IL-6 in children with MPP were significantly higher than those in children with non- MPP and healthy infants in the acute stag and the 4th week （ P〈0.05 ）, while the percentage of Th17 ceils and the levels of IL-17 and IL＇6 in children with non- MPP were significantly low in the 4th week, it was not significant difference between the children with MPP and the children with non-MPP. Conclusion： There is markedly mimunoresponsiveness unbalance in acute phase of MPP. Treg cell , Th17 ceils, IL-10, TGF-β , IL-17 and IL-6 may be involved in pathogenesis of MPP.

关 键 词：肺炎支原体肺炎 TREG细胞 TH17细胞 儿童 

分 类 号：R725.6[医药卫生—儿科]