Optimization of Extraction Process of Aflatoxin B_1 from Tartary Buckwheat Bran  

作　　者：Qiao LIN 

机构地区：[1]Xichang College

出　　处：《Agricultural Biotechnology》2015年第6期81-84,共4页农业生物技术（英文版）

基　　金：Supported by Key Science and Technology Project of Education Department of Sichuan Province(13ZA0157)

摘　　要：In this study, the extraction process of aflatoxin B~ from tartary buckwheat bran was optimized with ELISA detection method to determine the optimal conditions for extracting aflatoxin B1 from tartary buckwheat bran. The results of standard recovery test of blank solvent and sample confirmed the feasibility of ELISA detection method. Orthogonal experiment was performed to optimize the solid-liquid ratio, ultrasonic extraction time and ultrasonic amplitude. The results show that it is feasible to detect aflatoxin B1 content with ELISA method. The optimal ultrasonic extraction conditions were ： methanol-water ratio 6： 4, solld-liquid ratio 1 g： 5 ml, ultrasonic extraction time 15 min, ultrasonic amplitude 15 ~. Under the optimized conditions, 1 065.1 ng/L aflatoxin B1 was extracted from tartary buckwheat bran.In this study, the extraction process of aflatoxin B~ from tartary buckwheat bran was optimized with ELISA detection method to determine the optimal conditions for extracting aflatoxin B1 from tartary buckwheat bran. The results of standard recovery test of blank solvent and sample confirmed the feasibility of ELISA detection method. Orthogonal experiment was performed to optimize the solid-liquid ratio, ultrasonic extraction time and ultrasonic amplitude. The results show that it is feasible to detect aflatoxin B1 content with ELISA method. The optimal ultrasonic extraction conditions were ： methanol-water ratio 6： 4, solld-liquid ratio 1 g： 5 ml, ultrasonic extraction time 15 min, ultrasonic amplitude 15 ~. Under the optimized conditions, 1 065.1 ng/L aflatoxin B1 was extracted from tartary buckwheat bran.

关 键 词：Tartary buckwheat EXTRACTION Aflatoxin B1 ELISA 

分 类 号：Q946[生物学—植物学]