应用分子标记筛选辣椒雄性不育恢复系研究  

Screening of male sterile restorer line of pepper by molecular marker

作　　者：徐小万[1] 黄旺平[1] 吴智明[2] 李涛[1] 李颖[1] 王恒明[1] 

机构地区：[1]广东省农科院蔬菜研究所,广东广州510640 [2]仲恺农业工程学院园艺园林学院,广东广州510225

出　　处：《广东农业科学》2015年第23期139-144,共6页Guangdong Agricultural Sciences

基　　金：国家星火计划项目(2014GA780041);广东省科技计划项目(2013B020303008;2014A020208045);广州市科技计划项目(201510010119);广东省农业科学院蔬菜研究所所长基金(201404)

摘　　要：利用SCAR引物对359份未知育性的辣椒进行恢复基因的鉴定,结果显示,有35份材料有恢复基因特异条带,占比9.75%。恢复基因主要分布在小果型的辣味型品种和部分长果型(牛角形、羊角形)品种中。品种果实越大(横径越大)恢复基因越少,出现育性不稳定的比例也越高,即雄性不育系与亲缘关系较近的品种杂交易恢复,与亲缘关系较远的品种杂交易保持相一致。In this test, we selected 35 fertility restorers from 359 breeding materials using SCAR primer, accounting for 9.75% of the proportion of the total. Restorer gene mainly found in small fruit and elongated fruit. The larger the fruit diameter, the less the restorer gene was recovered, restorer gene appeared unstable sterility higher, It was easier for hybridization of male sterile line with close kinship varieties, with distant varieties, miscellaneous transactions remained consistent.

关 键 词：辣椒 胞质雄性不育系 恢复系 分子标记筛选 

分 类 号：S641.303.6[农业科学—蔬菜学]