抗菌药物氧氟沙星荧光增强及其荧光性质的研究  

Study on Fluorescence Enhancement and Fluorescence Properties of Antibacterial Drugs Ofloxacin

作　　者：杜梦圆[1] 杜浩然[1] 宋笛[1] 高蕻冰 李莹[1] 滕杰[2] 吕玉光[1] 

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯154007 [2]天津医科大学药学院天津市临床药物关键技术重点实验室,天津300070

出　　处：《广东化工》2016年第4期9-9,22,共2页Guangdong Chemical Industry

基　　金：国家自然科学基金项目(21346006);黑龙江省大学生创新创业训练计划项目(201510222035);黑龙江省教育厅科研项目(12541783);佳木斯大学交叉学科类科学研究项目(JC2014-005);佳木斯大学校长创新创业基金(xzyf2014-18);佳木斯大学研究生科技创新重点项目(LZR 2005_021)

摘　　要：提出一种基于荷移反应简便可靠地测定氧氟沙星(OFLX)的荧光光谱法,同时研究了电子受体四氰基乙烯(TCNE)与氧氟沙星电子给体之间的荷移络合物的光谱性质。确立了形成电荷转移(CT)络合物的最佳条件,在丙酮-甲醇混合介质中,于40℃水浴中恒温50 min即可形成1∶1络合物,其激发波长为331 nm。并对形成CT络合物的机理和检测抗菌药物氧氟沙星的方法进行了探讨。A Simple and reliable fluorescence spectrometric method for the determination of ofloxacin has been developed based on the charge-transfer reaction The charge-transfer complex which formed between ofloxacin as the donor and tetracyanoethylene as the acceptor has been studied by fluorimetry.It established the optimal conditions for forming a charge transfer（CT） complexes. In acetone-methanol mixed media, at 40 ℃ water bath heated 50 min to form 1∶1 complex excitation wavelength of 331 nm. At last, the charge-transfer reaction mechanism and detection of antimicrobial agents were discussed.

关 键 词：抗菌药物 氧氟沙星 四氰基乙烯 荧光性质 药物分析 

分 类 号：O657[理学—分析化学]